Comparison of Cytocompatibility Between Grit Blasted Titanium Alloy (Ti-6Al-4V) with or without Pure Titanium Coating.
- Author:
Juyoung KIM
1
;
Young Wook LIM
;
Soon Yong KWON
;
Yong Sik KIM
Author Information
1. Department of Orthopaedic Surgery, Incheon St. Mary's Hospital, Incheon, Korea.
- Publication Type:Original Article
- Keywords:
Ti alloy;
Pure titanium;
Electron beam deposition;
Cytocompatibility
- MeSH:
Alloys;
Aluminum;
Cell Line;
Cell Migration Assays;
Cell Proliferation;
Electrons;
Microscopy, Electron, Scanning;
Osteoblasts;
Spectrum Analysis;
Titanium;
Vanadium
- From:Journal of Korean Orthopaedic Research Society
2011;14(2):33-41
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: A composite of aluminum and vanadium (Ti-6Al-4V) is one of the most common compositions of titanium-based alloys. Unfortunately, vanadium has been found to cause adverse reactions. We evaluated the effects of vanadium containing titanium alloy (Ti-6Al-4V) on an osteoblast-like cell line (SaOS-2). MATERIALS AND METHODS: We studied the biologic and morphologic responses of SaOS-2 cell to Ti alloy with grit blasting and Ti coated Ti alloy with grit blasting. We performed energy-dispersive x-ray spectroscopy (EDS) and scanning electron microscopy (SEM) investigations and performed a cell proliferation assay, ALP activity, and cell migration assay of SaOS-2 cells. RESULTS: The morphologic assessment of cells through SEM showed that the two surfaces were covered with similar amounts of small slender osteoblast like cells. The amount of proliferation, ALP activity and the migration extent of SaOS-2 cells on the surfaces of each group were not statistically different. CONCLUSION: We used a grit-blasted Ti-coated Ti alloy, coated using electron beam deposition, and a grit-blasted Ti alloy to evaluate the toxicity of Ti-6Al-4V on SaOS-2 cell. Compared with pure titanium, the vanadium-containing Ti-alloy did not show an adverse effect on SaOS-2 cells.
