The effects of beta-TCP/rhBMP-2 on bone formation in osteoblast-like cells induced from bone marrow-derived mesenchymal stem cells.
- Author:
Yong Soo CHOI
1
;
Kyung Gyun HWANG
;
Jae Seon LEE
;
Chang Joo PARK
;
Kwang Sup SHIM
Author Information
1. Division of Oral and Maxillofacial Surgery / Department of Dentistry, College of Medicine, Hanyang University, Korea. hkg@hanyang.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
mesenchymal stem cell;
rhBMP -2;
beta-TCP;
differentiated osteoblast-like cell
- MeSH:
Alkaline Phosphatase;
Anthraquinones;
Ascorbic Acid;
Bone Morphogenetic Proteins;
Calcium;
Calcium Phosphates;
Dexamethasone;
Durapatite;
Glycerophosphates;
Humans;
Integrin-Binding Sialoprotein;
Mesenchymal Stromal Cells;
Osteoblasts;
Osteocalcin;
Osteogenesis;
RNA, Messenger;
Transcription Factors
- From:Journal of the Korean Association of Oral and Maxillofacial Surgeons
2008;34(4):419-427
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The present study aimed to investigate the osteogenic potentials of differentiated osteoblast-like cells (DOCs) induced from bone marrow-derived mesenchymal stem cells (MSCs) on beta-tricalcium phosphate (beta-TCP) with recombinant human bone morphogenetic protein (rhBMP-2) in vitro. Osteoblast differentiation was induced in confluent cultures by adding 100 nM dexamethasone, 10 mM beta-glycerophosphate, 50 mM L-ascorbic acid. The Alizarin red S staining and reverse transcriptase-polymerase chain reaction (RT-PCR) were perfomed to examine the mRNA expression of alkaline phosphatase (ALP), bone sialoprotein (BSP), osteocalcin (OCN), receptor activator for nuclear factor kappaB ligand (RANKL), runt-related transcription factor 2 (RUNX2), collagen-I (COL-I). There were no significant differences in the osteogenic potentials of DOCs induced from MSCs on beta-TCP(+/-). According to the incubation period, there were significant increasing of Alizadin red S staining in the induction 3 weeks. The mRNA expression of ALP, RUNX2, and RANKL were higher in DOCs/beta-TCP(-) than DOCs/beta-TCP(+). According to rhBMP-2 concentrations, the mRNA expression of BSP was significantly increased in DOCs/beta-TCP(+) compared to that of DOCs/beta-TCP(-) on rhBMP 10 ng/ml. Our study presented the beta-TCP will have the possibility that calcium phosphate directly affect the osteoblastic differentiation of the bone marrowderived MSCs.
