Detection of Infectious Bursal Disease Virus by Double Antibody Sandwich ELISA.
10.4167/jbv.2008.38.3.139
- Author:
Woo Jin JEON
1
;
Byung Sik CHANG
;
Mi Ja PARK
;
Eun Kyoung LEE
;
Hoo Don JOO
;
Jun Hun KWON
;
Kang Seuk CHOI
Author Information
1. National Veterinary Research Institute, National Veterinary Research and Quarantine ServiceAnyang, Gyeonggi, Korea. choiks@nvrqs.go.kr
- Publication Type:Original Article
- Keywords:
Antigen detection;
Diagnosis;
ELISA;
Infectious bursal disease
- MeSH:
Antibodies, Monoclonal;
Chickens;
Enzyme-Linked Immunosorbent Assay;
Immunosuppression;
Infectious bursal disease virus;
Limit of Detection;
Phenotype;
Poultry;
Viruses
- From:Journal of Bacteriology and Virology
2008;38(3):139-147
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Infectious bursal disease virus (IBDV) is responsible for a highly contagious disease of poultry causing severe immunosuppression in chickens. A double antibody sandwich ELISA (DAS-ELISA) was developed to detect IBDV from clinical samples. Two kinds of anti-IBDV antibodies, monoclonal antibody R63 and chicken anti-IBDV sera, were used for DAS-ELISA. Detection limit of IBDV by DAS-ELISA was approximately 10(2.7) EID(50)/ml. The DAS-ELISA detected IBDV from most (13/14) of vaccine products including mild, intermediate and intermediate-plus types. The DAS-ELISA also detected IBDV from all (19/19) of field Korean isolates including very virulent and intermediate-plus phenotypes. Our results indicate that the DAS-ELISA would provide useful diagnostic tool to detect IBDV from clinical samples as well as rapid quantitative detection of IBDV.
