Detection of Antibodies to Infectious Bursal Disease Virus (IBDV) by Agar Gel Immunodiffusion using Recombinant VP2 Protein.
10.4167/jbv.2008.38.3.149
- Author:
Woo Jin JEON
1
;
Byung Sik CHANG
;
Eun Kyoung LEE
;
Mi Ja PARK
;
Hoo Don JOO
;
Jun Hun KWON
;
Kang Seuk CHOI
Author Information
1. National Veterinary Research Institute, National Veterinary Research and Quarantine ServiceAnyang, Gyeonggi, Korea. choiks@nvrqs.go.kr
- Publication Type:Original Article
- Keywords:
AGID;
Antibody detection;
Infectious bursal disease;
VP2
- MeSH:
Agar;
Animals;
Antibodies;
Baculoviridae;
Bursa of Fabricius;
Chickens;
Enzyme-Linked Immunosorbent Assay;
Immunodiffusion;
Infectious bursal disease virus;
Korea;
Neutralization Tests;
Specific Pathogen-Free Organisms;
Sprains and Strains;
Staphylococcal Protein A;
Viruses
- From:Journal of Bacteriology and Virology
2008;38(3):149-159
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Infectious bursal disease virus (IBDV) causes a highly contagious and immunosuppressive disease of chicken. Agar gel immunodiffusion using IBDV antigen extracted from bursa of Fabricius of infected chicken has been used officially for diagnosis of IBDV in Korea. In this study, in order to replace the IBDV whole virus antigen with non-infectious antigen, recombinant VP2 protein (rVP2) of IBDV was produced using recombinant baculovirus expression system. Purified baculovirus-expressed rVP2 was used as an antigen in an agar gel immunodiffusion (AGID). rVP2 antigen precipitated specifically IBDV antibodies. AGID using rVP2 antigen detected anti-IBDV antibodies from 6 dpi to 28 dpi (termination of the experiment) when specific pathogen free chickens were experimentally infected with IBDV 52/70 strain. This was consistent with result by AGID using IBDV antigen, virus neutralization test (VNT) and a commercial ELISA kit (except for one serum). The sensitivity of rVP2 was the same with that of IBDV antigen when field sera (n=324) were tested by AGID. However, AGID using rVP2 antigen detected maternal antibodies from broiler chickens (n=20) on a broiler farm up to 15 days old, although the detection rate of the AGID was relatively low compared to a commercial ELISA kit. Our results indicate that IBDV whole virus antigen from IBDV infected chickens would be replaced with recombinant VP2 protein as an antigen for AGID.
