Simple Purification of Adeno-Associated Virus-DJ for Liver-Specific Gene Expression.
10.3349/ymj.2016.57.3.790
- Author:
Jingjing LIU
1
;
Young Ah MOON
Author Information
1. Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX, USA. yamoon15@inha.ac.kr
- Publication Type:Case Reports ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
- Keywords:
AAV-DJ;
large scale production;
liver-specific expression
- MeSH:
Animals;
Capsid;
Capsid Proteins/*genetics;
Dependovirus/*genetics;
*Gene Expression;
*Genetic Vectors;
Genome, Viral/genetics;
Hep G2 Cells;
Humans;
Liver/*metabolism;
Mice;
Mice, Inbred C57BL
- From:Yonsei Medical Journal
2016;57(3):790-794
- CountryRepublic of Korea
- Language:English
-
Abstract:
Recombinant gene expression using adeno-associated viruses (AAVs) has become a valuable tool in animal studies, as they mediate safe expression of transduced genes for several months. The liver is a major organ of metabolism, and liver-specific expression of a gene can be an invaluable tool for metabolic studies. AAV-DJ is a recombinant AAV generated by the gene shuffling of various AAV serotypes and shares characteristics of AAV2 and AAV8. AAV-DJ contains a heparin-binding domain in its capsid, which suggests that a heparin column could be used for the purification of the AAV. Given that AAV-DJ has been only recently available, relatively little is known about the optimal preparation/purification and application of AAV-DJ. Here, we present a simple large-scale preparation method that can generate 3×10(13) viral particles for in vivo experiments and demonstrate liver-specific gene expression via systemic injection in mice.
