Biocompatibility of two experimental scaffolds for regenerative endodontics.
- Author:
Dephne Jack Xin LEONG
1
;
Frank C SETZER
;
Martin TROPE
;
Bekir KARABUCAK
Author Information
- Publication Type:Original Article
- Keywords: Clindamycin; Matrix; Metronidazole; Regenerative endodontics; Revascularization; Scaffold
- MeSH: Apoptosis; Cell Proliferation; Clindamycin; Collagen; Dental Pulp; Endodontics*; Enzyme-Linked Immunosorbent Assay; Humans; Interleukin-8; Metronidazole; Microscopy; Regeneration; Stem Cells
- From:Restorative Dentistry & Endodontics 2016;41(2):98-105
- CountryRepublic of Korea
- Language:English
- Abstract: OBJECTIVES: The biocompatibility of two experimental scaffolds for potential use in revascularization or pulp regeneration was evaluated. MATERIALS AND METHODS: One resilient lyophilized collagen scaffold (COLL), releasing metronidazole and clindamycin, was compared to an experimental injectable poly(lactic-co-glycolic) acid scaffold (PLGA), releasing clindamycin. Human dental pulp stem cells (hDPSCs) were seeded at densities of 1.0 × 10(4), 2.5 × 10(4) and 5.0 × 10(4). The cells were investigated by light microscopy (cell morphology), MTT assay (cell proliferation) and a cytokine (IL-8) ELISA test (biocompatibility). RESULTS: Under microscope, the morphology of cells coincubated for 7 days with the scaffolds appeared healthy with COLL. Cells in contact with PLGA showed signs of degeneration and apoptosis. MTT assay showed that at 5.0 × 10(4) hDPSCs, COLL demonstrated significantly higher cell proliferation rates than cells in media only (control, p < 0.01) or cells co-incubated with PLGA (p < 0.01). In ELISA test, no significant differences were observed between cells with media only and COLL at 1, 3, and 6 days. Cells incubated with PLGA expressed significantly higher IL-8 than the control at all time points (p < 0.01) and compared to COLL after 1 and 3 days (p < 0.01). CONCLUSIONS: The COLL showed superior biocompatibility and thus may be suitable for endodontic regeneration purposes.
