The Expression of alpha2-Adrenergic Receptor Subtypes in the Central Nervous System of Peripheral Nerve Injured Rats.
10.4097/kjae.2006.51.4.468
- Author:
So Ron CHOI
1
;
Hyung Chang LEE
;
Chan Jong CHUNG
;
Hae Kyu KIM
Author Information
1. Department of Anesthesiology and Pain Medicine, College of Medicine, Dong-A University, Korea. cjchung@dau.ac.kr
- Publication Type:Original Article
- Keywords:
alpha(2)-adrenergic receptor;
neuropathic pain;
peripheral nerve injury;
mechanical allodynia
- MeSH:
Animals;
Central Nervous System*;
Hyperalgesia;
Hypothalamus;
Models, Animal;
Neuralgia;
Peripheral Nerve Injuries;
Peripheral Nerves*;
Rats*;
Real-Time Polymerase Chain Reaction;
RNA, Messenger;
Sciatic Nerve;
Thalamus
- From:Korean Journal of Anesthesiology
2006;51(4):468-475
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The change of expression of the alpha2-adrenergic receptor (alpha(2)-AR) subtypes in the thalamus and hypothalamus were investigated in a neuropathic pain rat model. METHODS: The left sciatic nerve was clamped for creating a neuropathic pain model in five rats. A sham operation was done in three rats as control group. Behavioral tests for pain were conducted by using mechanical stimuli applied to the hind paws. After 7 days, the expression of alpha(2)-AR subtype mRNA in the rat thalamus and hypothalamus was measured using real time polymerase chain reaction. RESULTS: Mechanical allodynia were developed on postoperative 1, 3, and 7 days in the neuropathic pain model. The expression of alpha(2A)-AR, alpha(2B)-AR, and alpha(2C)-AR was significantly higher in the thalamus and hypothalamus in the neuropathic pain model (P > 0.05). CONCLUSIONS: These results would suggest that the subtypes of alpha(2)-AR in thalamus and hypothalamus may contribute to produce neuropathic pain.
