The Protective Effect of Ischemic and Hypoxic Preconditioning on Hypoxic-ischemic Brain Injury in the Neonatal Rat: 1H Magnetic Resonance Spectroscopic Study.
10.4097/kjae.2006.50.2.188
- Author:
Sung Moon JEONG
1
;
Hwa Sung JUNG
;
Jae Moon CHOI
;
Ji Yeon BANG
;
Keun Ho LIM
;
Pyung Hwan PARK
Author Information
1. Department of Anesthesiology and Pain Medicine, College of Medicine, University of Ulsan, Asan Medical Center, Seoul, Korea. phpark@amc.seoul.kr
- Publication Type:Original Article
- Keywords:
1H magnetic resonance spectroscopic study;
hypoxic-ischemic brain injury;
hypoxic preconditioning;
ischemic preconditioning
- MeSH:
Animals;
Anoxia;
Apoptosis;
Aspartic Acid;
Brain Injuries*;
Brain Ischemia;
Brain*;
Carotid Artery, Common;
Ischemic Preconditioning;
Ligation;
Magnetic Resonance Spectroscopy;
Rats*;
Rats, Sprague-Dawley
- From:Korean Journal of Anesthesiology
2006;50(2):188-197
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: A brief episode of cerebral ischemia confers transient ischemic tolerance to a subsequent ischemic challenge. We examined the effect of ischemic and hypoxic preconditioning in the neonatal rat. METHODS: Seven-day old Sprague-Dawley rat pups were divided into three groups:control (n = 53), ischemic preconditioning (n = 51), and hypoxic preconditioning (n = 48). For ischemic preconditioning, the right common carotid artery was occluded for 10 min. Rats in the hypoxic preconditioning group were kept under hypoxic (8% oxygen/92% nitrogen) conditions for 4h. Twenty-four hours after the preconditioning, rats from all groups were exposed to the right common carotid artery ligature, followed by 2.5 h of hypoxia. Lipid/N-acetyl aspartate (Lip/NAA) and lipid/creatine (Lip/Cr) ratios from 1H MR spectroscopy and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) were evaluated as measures of apoptosis 1 and 7 days after hypoxic-ischemic injury. RESULTS: In the ischemic and hypoxic preconditioning groups, the Lip/NAA and Lip/Cr ratios and the numbers of TUNEL-positive cells were significantly lower than those in the control group (P < 0.05), but there were no significant differences between the two preconditioning groups. CONCLUSIONS: These results suggest that ischemic and hypoxic preconditioning in the neonatal rat attenuate the apoptosis that is caused by hypoxic-ischemic brain injury.
