Effect of xanthohumol on melanogenesis in B16 melanoma cells.
10.3858/emm.2008.40.3.313
- Author:
Jeung Hyun KOO
1
;
Hyoung Tae KIM
;
Ha Yong YOON
;
Kang Beom KWON
;
Il Whan CHOI
;
Sung Hoo JUNG
;
Han Uk KIM
;
Byung Hyun PARK
;
Jin Woo PARK
Author Information
1. Department of Biochemistry, Medical School and Institute for Medical Sciences, Chonbuk National University, Jeonju 561-756, Korea. jinwoo@chonbuk.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
cell differentiation;
melanins;
melanocytes;
microphthalmia-associated transcription factor;
monophenol monooxygenase;
xanthohumol
- MeSH:
1-Methyl-3-isobutylxanthine/pharmacology;
Animals;
Cell Line;
Cell Survival/drug effects;
Dose-Response Relationship, Drug;
Down-Regulation;
Drug Antagonism;
Forskolin/pharmacology;
*Humulus;
Intramolecular Oxidoreductases/antagonists & inhibitors/biosynthesis;
Melanins/antagonists & inhibitors/*biosynthesis;
Melanocytes/*drug effects/*metabolism;
Melanoma, Experimental;
Membrane Glycoproteins/antagonists & inhibitors/biosynthesis;
Mice;
Microphthalmia-Associated Transcription Factor/antagonists & inhibitors;
Monophenol Monooxygenase/antagonists & inhibitors/biosynthesis/genetics;
Oxidoreductases/antagonists & inhibitors/biosynthesis;
Propiophenones/*pharmacology;
Signal Transduction/drug effects;
alpha-MSH/metabolism
- From:Experimental & Molecular Medicine
2008;40(3):313-319
- CountryRepublic of Korea
- Language:English
-
Abstract:
Xanthohumol (XH), the principal prenylflavonoid of the hop plant (Humulus lupulus L.), dose-dependently inhibited isobutylmethylxanthine (IBMX)-induced melanogenesis in B16 melanoma cells, with little cytotoxicity at the effective concentrations. Decreased melanin content was accompanied by reduced tyrosinase enzyme activity, protein and mRNA expression. The levels of tyrosinase-related protein 1 and 2 mRNAs were decreased by XH. XH also inhibited alpha-melanocyte stimulating hormone- or forskolin-induced increases in melanogenesis, suggesting an action on the cAMP-dependent melanogenic pathway. XH downregulated the protein and mRNA expression of microphthalmia-associated transcription factor (MITF), a master transcriptional regulator of key melanogenic enzymes. These results suggest that XH might act as a hypo-pigmenting agent through the downregulation of MITF in the cAMP-dependent melanogenic pathway.
