Gene Expression Profile during Chondrogenesis in Human Bone Marrow derived Mesenchymal Stem Cells using a cDNA Microarray.
10.3346/jkms.2011.26.7.851
- Author:
Hyun Jung YOO
1
;
Sung Soo YOON
;
Seon Yang PARK
;
Eun Young LEE
;
Eun Bong LEE
;
Ju Han KIM
;
Yeong Wook SONG
Author Information
1. Department of Internal Medicine, Rheumatism Research Institute, Seoul National University College of Medicine, Seoul, Korea. ysong@snu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Chondrogenesis;
Mesenchymal Stem Cells;
cDNA Microarray
- MeSH:
Bone Marrow Cells/*cytology;
Cell Differentiation;
Chondrocytes/metabolism;
Chondrogenesis/*genetics;
*Gene Expression Profiling;
Humans;
Mesenchymal Stem Cells/cytology/*metabolism;
Oligonucleotide Array Sequence Analysis;
Reverse Transcriptase Polymerase Chain Reaction;
Time Factors
- From:Journal of Korean Medical Science
2011;26(7):851-858
- CountryRepublic of Korea
- Language:English
-
Abstract:
Mesenchymal stem cells (MSCs) have the capacity to proliferate and differentiate into multiple connective tissue lineages, which include cartilage, bone, and fat. Cartilage differentiation and chondrocyte maturation are required for normal skeletal development, but the intracellular pathways regulating this process remain largely unclear. This study was designed to identify novel genes that might help clarify the molecular mechanisms of chondrogenesis. Chondrogenesis was induced by culturing human bone marrow (BM) derived MSCs in micromass pellets in the presence of defined medium for 3, 7, 14 or 21 days. Several genes regulated during chondrogenesis were then identified by reverse transcriptase-polymerase chain reaction (RT-PCR). Using an ABI microarray system, we determined the differential gene expression profiles of differentiated chondrocytes and BM-MSCs. Normalization of this data resulted in the identification of 1,486 differentially expressed genes. To verify gene expression profiles determined by microarray analysis, the expression levels of 10 genes with high fold changes were confirmed by RT-PCR. Gene expression patterns of 9 genes (Hrad6B, annexinA2, BMP-7, contactin-1, peroxiredoxin-1, heat shock transcription factor-2, synaptotagmin IV, serotonin receptor-7, Axl) in RT-PCR were similar to the microarray gene expression patterns. These findings provide novel information concerning genes involved in the chondrogenesis of human BM-MSCs.
