Anticancer effect of CKD-602(belotecan, camtobell(R)) on the oral cancer cell lines.
- Author:
Pil Young YUN
1
;
Yong Ju OK
;
Hoon MYOUNG
;
Jong Ho LEE
;
Myung Jin KIM
Author Information
1. Department of Oral and Maxillofacial Surgery, College of Dentistry, Seoul National University, Korea. myungkim@plaza.snu.ac.kr
- Publication Type:Original Article
- Keywords:
CKD-602;
Camptothecin;
Topoisomerase-I inhibitor;
Oral cancer;
Apoptosis
- MeSH:
Apoptosis;
Camptothecin;
Cell Death;
Cell Line*;
Colonic Neoplasms;
DNA Topoisomerases, Type I;
Lung Neoplasms;
Mouth Neoplasms*
- From:Journal of the Korean Association of Oral and Maxillofacial Surgeons
2005;31(1):7-12
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: CKD-602, a newly developed water-soluble campthotecin analogue, is a anticancer agent which act as a DNA topoisomerase I inhibitor. CKD-602 is known as more potent and tolerable agent. The main purposes of this study were to measure the cytotoxic effect of CKD-602 on the oral cancer cell lines and to evaluate the apoptotic aspect of dead cells. MATERIALS AND METHODS: To determine the cytotoxic effect of CKD-602 on the oral cancer cell lines in comparison with various cell lines, such as lung cancer and colon cancer cell lines, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay was performed. And apoptosis was analyzed using fluorescence-activated cell sorting(FACS) system. RESULTS: CKD-602 decreased the viability of malignant cells in a dose dependent manner and in a time dependent manner. CKD-602 showed excellent cytotoxicity to the oral cancer cell lines. Also, apoptotic portion was increased in a dose dependent manner. CONCLUSION: These findings indicated that CKD-602 induced apoptotic cell death in the various cell lines including oral cancer cell lines. From the results, it was suggested that CKD-602 would be a potential therapeutic agent for the oral cancer. More successive researches on the anticancer effect of CKD-602 should be performed.
