Study on Changes in Endogenous Stem Cells in the Salivary Gland of Streptozotocin-induced Diabetic Rats.
10.11620/IJOB.2017.42.3.099
- Author:
Bo Hyun JUNG
1
;
Hee Su LEE
;
Ki Yeon YOO
Author Information
1. Department of Anatomy, College of Dentistry and Research Institute of Oral Science, Gangneung-Wonju National University, Gangneung, Korea. kyyoo@gwnu.ac.kr
- Publication Type:Original Article
- Keywords:
Diabetes;
Salivary gland;
Stem cell;
Regeneration;
Xerostomia
- MeSH:
Animals;
Apoptosis;
Brain;
Cell Death;
Cell Proliferation;
Diabetes Mellitus;
Immunohistochemistry;
In Situ Nick-End Labeling;
Polydipsia;
Rats*;
Regeneration;
Salivary Glands*;
Stem Cells*;
Xerostomia
- From:International Journal of Oral Biology
2017;42(3):99-106
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Type1 diabetes mellitus (DM) is generally known to be caused by destruction of insulin-producing pancreatic β cells or an immune-related problem. Polydipsia is a representative symptom of DM, and it has been reported that this condition is closely related to xerostomia and is considered that hyposalivation from the salivary gland results in this phenomenon. Although various studies have reported that induction of diabetes reduces endogenous stem cells in other organs (heart, brain etc.), diabetes-related changes in endogenous stem cells in the salivary gland have not yet been well established. Therefore, in this study, to verify the change in salivary gland stem cells after diabetes, salivary gland tissues in the control and diabetes-induced groups were processed by histochemistry (Masson's trichrome staining) for morphological analysis, TUNEL assay for cell death, and immunohistochemistry (Ki-67 and c-Kit) for cell proliferation and maturation. Diabetes induced by STZ leads to vacuolization, apoptosis, and reduction in proliferating cells/salivary gland stem cells in salivary glands of rats. This result suggests that diabetes may be associated with reduction in salivary gland function such as degeneration and inhibition of regeneration in the salivary gland.
