Mixture Patterned Short Tandem Repeat Profile in a Perimortem Transfused Patient.
10.7580/kjlm.2016.40.1.27
- Author:
Hee Jin SEO
1
;
Sohee CHO
;
Ji Hyun LEE
;
Sung Hee LYOO
;
Sohyung PARK
;
Soong Deok LEE
Author Information
1. Department of Forensic Medicine, Seoul National University College of Medicine, Seoul, Korea. sdlee@snu.ac.kr
- Publication Type:Case Report
- Keywords:
Perimortem;
Blood transfusion;
DNA profiling;
Microsatellite repeats
- MeSH:
Autopsy;
Blood Transfusion;
DNA;
DNA Fingerprinting;
Humans;
Microsatellite Repeats*;
Tissue Donors
- From:Korean Journal of Legal Medicine
2016;40(1):27-31
- CountryRepublic of Korea
- Language:English
-
Abstract:
Recently, it has been reported that transfused patients can generate admixture-like genetic profiles. As genetic material of the donor can survive for a reasonable time after transfusion, the recipient's genomic DNA is likely to have a mixture pattern. An autopsy case of a man transfused perimortem generated a mixture patterned short tandem repeat profile. Notably, the patient was transfused mostly with nuclear-deficient cells, limiting the donor genetic material available for the recipient. As a result, mixture-like patterns were observed consistently, regardless of change in input DNA content; the sample DNA content, which was serially diluted, ranged from 1 ng to 0.0625 ng. The distributions of foreign peaks appeared to be irreproducible, showing stochastic behaviors throughout the genotyped results. This study suggests that a cautious approach is required when genotyping of a patient who has undergone recent transfusion. One must consider the possibility of obtaining a mixture patterned profile in such patients, and therefore, choose parenchymal organs or tissues for reliable results.
