Genotype Analysis of Granulocyte-specific Antigens in Koreans.
- Author:
Dong Hee SEO
;
Sung Sup PARK
;
Kyou Sup HAN
- Publication Type:Original Article
- MeSH:
Acute Lung Injury;
Asian Continental Ancestry Group;
Autoantibodies;
DNA;
DNA Fingerprinting;
Eosinophils;
Gene Frequency;
Genotype*;
Humans;
Isoantibodies;
Neutropenia;
Neutrophils;
Phenotype;
Polymerase Chain Reaction;
Serotyping
- From:Korean Journal of Clinical Pathology
1997;17(6):1144-1149
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The granulocyte-specific antigen NA which exist specifically on neutrophils and eosinophils have its antigenic determinant on Fcgamma receptor III. The alloantibodies and autoantibodies against this antigen are responsible for neonatal alloimmune neutropenia, chronic benign autoimmune neutropenia of infancy and transfusion-related acute lung injury. After discovering of biallelic polymorphism of NA antigen, there have been attempts to replace technically cumbersome serotyping methods by DNA-typing. This study was performed to investigate the genotype frequency of NA antigen In Koreans by DNA typing. METHODS: Using genomic DNA extracted from venous blood of 200 Koreans, genotype of NA antigen system was determined through polymerase chain reaction with sequence-specific primers (PCR-SSP), and the frequency was compared with previous studies. The NA genotypes of seven individuals were compared with their known phenotypes. RESULTS: The genotype frequencies of NA1+NA2-, NA1+NA2+, and NA1-NA2+ were 28.5%, 59.0%, and 12.5% respectively. The gene frequencies calculated from genotype frequencies were 0.58 and 0.42 for NA1 and NA2 respectively. The NA genotypes of seven individuals were correlated precisely with their phenotypes. CONCLUSION: The gene frequency of NA1 in Koreans was higher than that of Caucasian. The genotype frequencies of NA antigen in Koreans were similar to those of the Japanese and Chinese. This study will serve as a basic data for the study of the patients with diseases associated with granulocyte-specific antigens in Koreans.
