Quantitative Analysis of Transforming Growth Factor-beta1 in Renal Cell Carcinoma.
- Author:
Bong Ryoul OH
1
;
Sung Jin KIM
;
Jae Gue LEE
;
Dong Deuk KWON
;
Soo Bang RYU
;
Yang Il PARK
Author Information
1. Departments of Urology and 1Dermatology, Chonnam National University Medical School, Gwangju, Korea.
- Publication Type:Original Article
- Keywords:
Transforming growth factor-beta1;
Renal cell carcinoma;
Enzyme-linked immunosorbent assay
- MeSH:
Carcinogenesis;
Carcinoma, Renal Cell*;
Cytoplasm;
Enzyme-Linked Immunosorbent Assay;
Humans;
Kidney;
Transforming Growth Factor beta1
- From:Korean Journal of Urology
2002;43(3):197-201
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Purpose: The transforming growth factor-beta (TGF-beta) is a peptide that has diverse biologic actions in human tissue and is thought to contribute to tumor development and progression. Increased TGF-beta1 levels were found in several types of malignant tumors. TGF-beta1 expression in RCC and adjacent normal kidney tissues was examined to determine the TGF-beta1 in renal cell carcinoma (RCC). MATERIALS AND METHODS: The TGF-beta1 protein levels in cancer and a normal portion of a specimen were analyzed in 61 radical nephrectomized clear cell type RCC by an enzyme-linked immunosorbent assay (ELISA), with the results compared with the clinicopathological characteristics. Immunohistochemical staining was performed to localize their expression. RESULTS: Compared with non-tumor kidney specimens, primary renal cell carcinoma demonstrated a significantly higher TGF-beta1 protein level (p<0.001). There were significant differences in the TGF-beta1 level among the histological grade (p<0.01). The tissue TGF-beta1 level was the highest in T4 stage, but there was no statistical significance between the T stages. Immunohistochemical analysis demonstrated that TGF-beta1 was localized to the tumor cytoplasm and their intensity reflected the protein expression level in these tissues. CONCLUSIONS: These results suggest that enhanced TGF-beta1 expression contributes to carcinogenesis and tumor progression in the later stages of renal cell carcinoma.
