Comparative study of the immunological characteristics of three different C57BL/6N mouse substrains.
10.5625/lar.2017.33.2.124
- Author:
Jun Young CHOI
1
;
Hye Ryeong KIM
;
Kil Soo KIM
;
Young Suk JUNG
;
Joon Young CHO
;
Dae Youn HWANG
;
Hyun Keun SONG
Author Information
1. Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Korea. dyhwang@pusan.ac.kr
- Publication Type:Comparative Study ; Original Article
- Keywords:
C57BL/6N;
C57BL/6NKorl;
immune cell;
spleen;
thymus
- MeSH:
Adaptive Immunity;
Allergy and Immunology;
Allografts;
Animals;
B-Lymphocytes;
Flow Cytometry;
Granulocytes;
Immunity, Innate;
Isoantigens;
Killer Cells, Natural;
Lymphocytes;
Macrophages;
Major Histocompatibility Complex;
Mice*;
Spleen;
Thymus Gland;
Weights and Measures
- From:Laboratory Animal Research
2017;33(2):124-131
- CountryRepublic of Korea
- Language:English
-
Abstract:
Inbred mice, a systematically developed homogeneous animal, have been developed to maintain experimental reproducibility and to minimize experimental variables in animal-based studies. In particular, C57BL/6 mice are frequently used in experiments into immunology and antitumor activity experiments. This study was compared the immunological characteristics of C57BL/6NKorl, a Korean developed experimental animal resource, with those of two other C57BL/6N substrains. Mouse body, thymus, and spleen weights in C57BL/6NKorl were not significantly different from those of the other two C57BL/6N substrains. Among the three substrains, there was no difference in the distribution of T and B cells, which are lymphocytes involved in adaptive immunity, and no difference in NK cells related to innate immunity. Results for macrophages and granulocytes, which have roles in innate immunity, were similar in all three substrains. In order to investigate the expressions of major histocompatibility complex (MHC) molecules and allogenic antigens, splenocytes were separated from obtained spleen and analyzed by using flow cytometry. MHC class I and II molecules, which are important during self/non-self-discrimination, were similar in the three substrains. In addition, expression of alloantigen involved in allografts showed similar results in the three substrain. Thus, the results of this study provide strong evidence that C57BL/6NKorl is immunologically similar to two other C57BL/6N substrains.
