Comparison of humoral and cell-mediated immunity in three different C57BL/6N mouse substrains.
10.5625/lar.2017.33.2.132
- Author:
Hye Ryeong KIM
1
;
Jun Young CHOI
;
Kil Soo KIM
;
Young Suk JUNG
;
Joon Young CHO
;
Dae Youn HWANG
;
Hyun Keun SONG
Author Information
1. Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Korea. dyhwang@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
C57BL/6N;
C57BL/6NKorl;
T cell;
B cell;
cell-mediated immunity;
humoral immunity
- MeSH:
Adaptive Immunity;
Animals;
B-Lymphocytes;
Cell Count;
Immunity, Cellular*;
Immunity, Humoral;
Memory;
Mice*;
Spleen;
T-Lymphocytes;
Thymus Gland
- From:Laboratory Animal Research
2017;33(2):132-139
- CountryRepublic of Korea
- Language:English
-
Abstract:
Adaptive immunity is a type of immune response mediated by T and B cells, and is important response for immune response amplification and memory. In this study, the adaptive immunologic properties of C57BL/6NKorl substrain were compared with those of two other C57BL/6N substrains. There were no significant differences between the C57BL/6NKorl and the two other C57BL/6N substrains in the histological structures of the thymus and spleen, which are immunologic organs containing T cell and B cells. In addition, flow cytometric analysis did not reveal any significant differences in the distribution of T and B cell populations of the three substrains. To evaluate cell-mediated immunity of T cells in the three different substrains, we treated isolated T cells from spleen with Con A. The T cells of C57BL/6NKorl showed Con A-dependent proliferation of T cells at lower cell number than those in T cells from the other two C57BL/6N substrains. B cell-mediated humoral immune responses were not significant different among the three substrains. Thus, the results of this study provide evidence that C57BL/6NKorl mice are similar to those two other C57BL/6N substrains in humoral immunity, but C57BL/6NKorl has stronger response in cell mediated immunity.