Cordycepin Suppresses Expression of Diabetes Regulating Genes by Inhibition of Lipopolysaccharide-induced Inflammation in Macrophages.
- Author:
Seulmee SHIN
1
;
Sungwon LEE
;
Jeonghak KWON
;
Sunhee MOON
;
Seungjeong LEE
;
Chong Kil LEE
;
Kyunghae CHO
;
Nam Joo HA
;
Kyungjae KIM
Author Information
- Publication Type:Original Article
- Keywords: cordycepin; type 2 diabetes; pro-inflammatory cytokines; immunomodulator
- MeSH: 11-beta-Hydroxysteroid Dehydrogenase Type 1; Agaricales; Blotting, Western; Cordyceps; Cytokines; Deoxyadenosines; Immune System; Inflammation; Intercellular Adhesion Molecule-1; Interleukin-6; Macrophages; Metabolic Diseases; NF-kappa B; PPAR gamma; RNA, Messenger; Tumor Necrosis Factor-alpha
- From:Immune Network 2009;9(3):98-105
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: It has been recently noticed that type 2 diabetes (T2D), one of the most common metabolic diseases, causes a chronic low-grade inflammation and activation of the innate immune system that are closely involved in the pathogenesis of T2D. Cordyceps militaris, a traditional medicinal mushroom, produces a component compound, cordycepin (3'-deoxyadenosine). Cordycepin has been known to have many pharmacological activities including immunological stimulating, anti-cancer, and anti-infection activities. The molecular mechanisms of cordycepin in T2D are not clear. In the present study, we tested the role of cordycepin on the anti-diabetic effect and anti-inflammatory cascades in LPS-stimulated RAW 264.7 cells. METHODS: We confirmed the levels of diabetes regulating genes mRNA and protein of cytokines through RT-PCR and western blot analysis and followed by FACS analysis for the surface molecules. RESULTS: Cordycepin inhibited the production of NO and pro-inflammatory cytokines such as IL-1beta, IL-6, and TNF-alpha in LPS-activated macrophages via suppressing protein expression of pro-inflammatory mediators. T2D regulating genes such as 11beta-HSD1 and PPARgamma were decreased as well as expression of co-stimulatory molecules such as ICAM-1 and B7-1/-2 were also decreased with the increment of its concentration. In accordance with suppressed pro-inflammatory cytokine production lead to inhibition of diabetic regulating genes in activated macrophages. Cordycepin suppressed NF-kappaB activation in LPS-activated macrophages. CONCLUSION: Based on these observations, cordycepin suppressed T2D regulating genes through the inactivation of NF-kappaB dependent inflammatory responses and suggesting that cordycepin will provide potential use as an immunomodulatory agent for treating immunological diseases.
