Effects of HMGB-1 Overexpression on Cell-Cycle Progression in MCF-7 Cells.
10.3346/jkms.2004.19.3.321
- Author:
Sarah YOON
1
;
Jin Young LEE
;
Byung Koo YOON
;
Duk Soo BAE
;
Doo Seok CHOI
Author Information
1. Center for Clinical Medicine & Samsung Biomedical Research Institute, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Cell Cycle;
HMG-Box Domains;
HMGB1 Protein;
Receptors, Estrogen
- MeSH:
Blotting, Western;
Cell Cycle;
Cell Line, Tumor;
Densitometry;
Estrogens/metabolism;
G2 Phase;
Genetic Vectors;
HMGB1 Protein/*biosynthesis;
Human;
Kinetics;
Oligonucleotides/chemistry;
Plasmids/metabolism;
Protein Structure, Tertiary;
RNA, Messenger/metabolism;
Reverse Transcriptase Polymerase Chain Reaction;
S Phase;
Support, Non-U.S. Gov't;
Time Factors;
Trans-Activation (Genetics);
Transfection
- From:Journal of Korean Medical Science
2004;19(3):321-326
- CountryRepublic of Korea
- Language:English
-
Abstract:
High mobility group-1 (HMGB-1) enhances the DNA interactions and possesses a transcriptional activation potential for several families of sequence-specific transcriptional activators. In order to examine the effect of HMGB-1 on the cell cycle progression in MCF-7 cells, the HMGB-1 expression vector was transfected into synchronized MCF-7 cells, and the effect of HMGB-1 overexpression on the cell cycle was examined. The HMGB-1 protein level in the transfected cells increased 4.87-fold compared to the non-transfected cells. There were few changes in the cell cycle phase distribution after HMGB-1 overexpression in the MCF-7 cells. Following the estrogen treatment, the cell cycle progressed in both the HMGB-1 overexpressed MCF-7 and the mock-treated cells. However, a larger proportion of HMGB-1 overexpressing MCF-7 cells progressed to the either S or G2 phase than the mock-treated cells. The mRNA levels of the cell cycle regulators changed after being treated with estrogen in both the HMGB-1 overexpressing MCF-7 and the mock-treated cells, but the changes in the expression level of the cell cycle regulator genes were more prominent in the HMGB-1 overexpressing MCF-7 cells than in the mock-treated cells. In conclusion, HMGB-1 overexpression itself does not alter the MCF-7 cell cycle progression, but the addition of estrogen to the HMGB-1 overexpressing MCF-7 cells appears to accelerate the cell cycle progression.
