Expression of Cell Cycle Regulators During Smooth Muscle Cell Proliferation After Balloon Catheter Injury of Rat Artery.
10.3346/jkms.2004.19.3.327
- Author:
Jung Kee CHUNG
1
;
Taeseung LEE
;
In Mok JUNG
;
Young Kyun KIM
;
Seung Kee MIN
;
Jeong Wook SUH
;
Sang Joon KIM
Author Information
1. Department of Surgery, Seoul National University College of Medicine, Seoul, Korea. sjkimgs@plaza.snu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Endothelium, Vascular;
Myocytes, Smooth Muscle;
Tunica Intima;
Cell Cycle Proteins;
Rat
- MeSH:
Animals;
Arteries/*pathology;
Balloon Dilatation/*adverse effects;
Blotting, Western;
CDC2-CDC28 Kinases/biosynthesis;
Cell Cycle;
Cell Cycle Proteins/biosynthesis;
Cell Division;
Cyclin E/biosynthesis;
Cyclins/biosynthesis;
Endothelium, Vascular/pathology;
Extracellular Matrix/metabolism;
Hyperplasia/pathology;
Iliac Artery/pathology;
Immunohistochemistry;
Male;
Myocytes, Smooth Muscle/*cytology;
Proliferating Cell Nuclear Antigen/biosynthesis;
Rats;
Rats, Sprague-Dawley;
Support, Non-U.S. Gov't;
Time Factors;
Tumor Suppressor Proteins/biosynthesis
- From:Journal of Korean Medical Science
2004;19(3):327-332
- CountryRepublic of Korea
- Language:English
-
Abstract:
Intimal hyperplasia is defined as the abnormal migration and proliferation of vascular smooth muscle cells (VSMCs) with deposition of extracellular matrix. However, the cell cycle regulatory mechanisms of injury-induced VSMC proliferation are largely unknown. To examine the expression kinetics of cell cycle regulatory factors which is known to be worked positively or negatively, we used rat balloon injury model. Marked induction of proliferating cell nuclear antigen (PCNA), G1/S cyclin-dependent kinase (cdk2), and its regulatory subunit (cyclin E) occurred between 1 and 3 days after balloon arterial injury, and this was sustained for up to 7 days and then declined. However, the induction of the negative regulators, p21 and p27, occurred between 3 and 5 days of injury, peaked after 7 and 14 days and was then sustained. VSMC proliferation after balloon catheter injury of the rat iliac artery is associated with coordinated expression of positive (cdk2, cyclin E and PCNA) and negative (p21, p27) regulators. Cell cycle regulators such as cdk2, cyclin E, p21, p27 may be suitable targets for the control of intimal hyperplasia.
