Effect of CD14, Toll-like receptors, cytoskeletal inhibitors and NF-kappaB inhibitor on MMP-8 release from human neutrophils induced by E. coli lipopolysaccharides.
10.5051/jkape.2005.35.2.427
- Author:
Seung Min YANG
1
;
Tae Il KIM
;
Yang Jo SEOL
;
Yong Moo LEE
;
Young KU
;
Chong Pyoung CHUNG
;
Soo Boo HAN
;
In Chul RHYU
Author Information
1. Department of Periodontology, School of Dentistry, Seoul National University, Korea. icrhyu@snu.ac.kr
- Publication Type:Original Article
- Keywords:
Human Neutrophil;
CD14;
TLRs;
Cytoskeletal Inhibitors;
NF-B inhibitor;
MMP-8
- MeSH:
Actin Cytoskeleton;
Antibodies;
Cytokines;
Enzyme-Linked Immunosorbent Assay;
Humans*;
Lipopolysaccharides*;
Microtubules;
Neutrophils*;
NF-kappa B*;
Periodontal Diseases;
Polymerization;
Polymers;
Signal Transduction;
Tissue Donors;
Toll-Like Receptors*;
Tosylphenylalanyl Chloromethyl Ketone
- From:The Journal of the Korean Academy of Periodontology
2005;35(2):427-436
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: MMP-8 is a neutrophil enzyme and its level increases in some inflammatory diseases, including periodontal disease. We knew that the lipopolysaccharide of E.coli(E-LPS) induced MMP-8 release from human neutrophils. E-LPS is known to induce the production and release of inflammatory cytokines through CD14, Toll-like receptor(TLR). In the present study, we investigated whether MMP-8 release by E-LPS is induced via CD14-TLR pathway and the cellular mechanism of MMP-8 release in human neutrophils. MATERIAL AND METHODS: Human neutrophils were isolated from the peripheral blood of healthy donors and pre-incubated in medium containing antibodies against CD14, anti-TLR2 and anti-TLR4 or several inhibitors of microtubules and microfilaments and then incubated with E-LPS. The cells were treated TPCK and E-LPS simultaneously. The MMP-8amount in the culture medium was determined using ELISA. RESULTS: E-LPS increased MMP-8release from neutrophils and its induction was inhibited by anti- CD14 and anti-TLR4 but not by anti-TLR2 antibodies. The inhibitors of micro- tubule and microfilament polymerization significantly decreased E-LPS-induced MMP- 8release. TPCK inhibited E-LPS-induced MMP-8 release. CONCLUSION: These results suggest that MMP-8 release is induced by E-LPS via the CD14-TLR4 signal pathway in human neutrophils and may be depedent on microtubule and microfilament systems and NF-kappaB pathway.
