Osteogenic activity of an adenovirus expressing BMP-2 on Human Periodontal Ligament cells.
10.5051/jkape.2005.35.2.511
- Author:
Kyoung Hwa KIM
1
;
Yoon Jeong PARK
;
Sang Cheul LEE
;
Tae Il KIM
;
Yang Jo SEOL
;
Yong Moo LEE
;
Young KU
;
Soo Boo HAN
;
Chong Pyoung CHUNG
;
In Chul RHYU
Author Information
1. Department of Periodontology, School of Dentistry, Seoul National University, Korea. icrhyu@snu.ac.kr
- Publication Type:Original Article
- Keywords:
BMP-2;
Adenovirus;
PDL cell;
gene delivery;
collagen scaffold
- MeSH:
Adenoviridae*;
Biological Availability;
Collagen;
Connective Tissue;
Dental Cementum;
Humans*;
Periodontal Ligament*;
Regeneration;
Tooth;
Wounds and Injuries
- From:The Journal of the Korean Academy of Periodontology
2005;35(2):511-524
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The regeneration of lost periodontal tissue is a major goal of therapy. Periodontal ligament cell(PDL) is a specialized connective tissue that connects cementum and alveolar bone to maintain and support teeth in situ and preserve tissue homoeostasis. Bone morphogenetic proteins(BMPs) have shown much potential in the reconstruction of the periodontum by stimulate new bone and new cementum formation. Limitiations of BMP administration to periodontal lesions is high dose delivery, BMP transient biological activity, and low bioavailability of factors at the wound site. Gene delivery method can be alternative treatment strategy to deliver BMPs to periodontal tissue. The purpose of this study is to investigate efficiency of BMP-2 gene delivery with cell-based therapy using PDL cells. PDL cell were transduced with adenoviruses encoding either BMP-2 or Lac-Z gene. To evaluate osteogenic activity of expressed BMP-2 on PDL cells, we investigated secreted BMP-2, cellular activity, ALPase, produced mineralized nodules. To evaluate collagen scaffold as carrier for transduced cell delivery, we examined morphology and secreted BMP-2 of transducd PDL cells on it. BMP-2 transducd PDL cells produced higher levels of BMP-2, ALPase, mineralized nodules than non transduced cells. Cellular activity of transduced cells was showed similar activity to non transduced cells. Transduce cells attached on collagen scaffold secreted BMP-2 at 7day and was showed similar morphology to non transduced cells. These results demonstrated that transduced PDL cells produced biologically active BMP-2 and collagen scaffold could be carrier of transducd cells.
