Determination of the Biogroup of Vibrio vulnificus Isolates from Patients and Oysters in Korea.
- Author:
Phil Seung SEO
1
;
Bo Suk KIM
;
Shin Moo KIM
;
Seok Don PARK
Author Information
1. Department of Dermatology, Wonkwang University School of Medicine, Korea. sdpark@wku.ac.kr
- Publication Type:Original Article
- Keywords:
Biogroup;
Virio vulnificus
- MeSH:
Eels;
Humans;
Korea;
Ostreidae;
Polymerase Chain Reaction;
Vibrio;
Vibrio vulnificus
- From:Korean Journal of Dermatology
2008;46(11):1453-1456
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Vibrio vulnificus are divided into 3 biogroups based on their biochemical and serological properties and the existence of eel virulence. Only a few studies can be found on the biogroups of V. vulnificus in other countries, and no such studies have been done in Korea. OBJECTIVE: The aim of this study was to determine the presence of biogroups other than biogroup 1 among the V. vulnificus isolated from septic patients and from oysters in Korea. METHODS: A total of 103 isolates (53 from septic patients and 50 from oyster) were used. The API 20E system was used to confirm identification of the V. vulnificus. Conventional biochemical tests and vvA gene detection by polymerase chain reaction (PCR) were used to determine the biogroups. RESULTS: The clinical and oyster isolates showed results similar to the biochemical tests. All of the clinical and oyster isolates showed the biochemical pattern of biogroup 1. The vvhA gene was detected in all of the isolates. CONCLUSION: All of the V. vulnificus isolates from the septic patients and oysters in Korea belong to biogroup 1.
