Polymorphism of Methylenetetrahydrofolate Reductase Gene and the Risk of Childhood Acute Lymphoblastic Leukemia.
- Author:
Soo Jin YOO
1
;
Eun Heui SHIM
;
So Young KANG
;
Seongsoo JANG
;
Eul Ju SEO
;
Chan Jeoung PARK
;
Hyun Sook CHI
Author Information
1. Department of Laboratory Medicine, Gachon Medical School, Gil Medical Center, Incheon, Korea.
- Publication Type:Original Article
- Keywords:
Methylenetetrahydrofolate reductase;
Polymorphism;
Acute lymphoblastic leukemia
- MeSH:
Bone Marrow;
Genotype;
Humans;
Karyotype;
Methylenetetrahydrofolate Reductase (NADPH2)*;
Precursor Cell Lymphoblastic Leukemia-Lymphoma*
- From:The Korean Journal of Laboratory Medicine
2005;25(6):379-384
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The polymorphisms, C677T and A1298C in the methylenetetrahydrofolate reductase (MTHFR) gene express a decreased enzyme activity. These polymorphic variants are known to decrease the risk of some malignancies. We examined whether the polymorphisms in MTHFR gene play a role in childhood acute lymphoblastic leukemia (ALL). METHODS: Peripheral blood or bone marrow samples were collected from 99 ALL patients aged <16 years and 105 age and sex matched controls. We performed PCR-restriction fragment length polymorphism with HinF1 for C677T and MboII A1298C. RESULTS: The frequencies of 677CC, 677CT, and 677TT genotypes were 43.4%, 46.5% and 10.1% in patients and 39.0%, 45.7%, and 15.2% in controls. The 677TT variants seemed to decrease the risk for ALL than the 677CC genotype, but the difference was not statistically significant (OR=0.60, 95% CI=0.2-1.5). The frequencies of 1298AA, 1298AC, and 1298CC genotype were 66.7%, 26.3% and 7.1% in patients and 60.0%, 38.1% and 1.9% in controls. The 1298CC genotype seemed to increase the risk for ALL than 1298AA, but without statistical significance (OR=3.34, 95% CI=0.7- 18.1). These findings were more evident in the patient groups with hyperploid and translocation than those with normal karyotypes. CONCLUSIONS: In our series, MTHFR C677T and A1298C polymorphism did not show a statistically significant protective effect for the childhood ALL.
