Evaluation of the detectability of Vitek II System for Inducible Clindamycin Resistance in Staphylococci.
- Author:
Sung Hyun LEE
1
;
Sook Jin JANG
;
Dae Soo MOON
;
Young Jin PARK
;
Gyun Yeol AHN
;
Hu Lin HAN
;
Bidur Prasad CHAULAGAIN
;
Ok Yeon JEONG
Author Information
1. Department of Laboratory Medicine, College of Medicine, Chosun University, Gwangju, Korea. sjbjang@chosun.ac.kr
- Publication Type:Original Article
- Keywords:
Inducible clindamycin resistance;
Staphylococcus aureus;
Vitek II;
Disk approximation test
- MeSH:
Clindamycin*;
Diffusion;
Erythromycin;
Methicillin;
Methicillin Resistance;
Methicillin-Resistant Staphylococcus aureus;
Staphylococcus aureus
- From:The Korean Journal of Laboratory Medicine
2005;25(6):406-410
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: While broth based antimicrobial susceptibility test methods work well for the detection of the majority of antimicrobial resistance mechanisms, antimicrobial resistance mechanism in some microorganisms may not be detected by these methods. The purpose of this study was to compare Vitek II system with a standard method for the ability to detect inducible clindamycin resistance in Staphylococcus aureus. METHODS: Of 200 clinical isolates of S. aureus tested, 183 were methicillin resistant (MRSA) and 17 were methicillin susceptible (MSSA). A disk approximation test (Clinical Laboratory Standards Institute; CLSI, Wayne, PA, USA) was performed as the standard method by placing standard erythromycin and clindamycin disks in adjacent positions. Vitek II ID-GPI (bioMerieux, Durham, NC, USA) was used for identification and Vitek AST-P536 (bioMerieux, Durham, NC, USA) for antimicrobial susceptibility tests. RESULTS: Clindamycin resistance rates of S. aureus tested by disk diffusion and Vitek II system were 89% and 56%, respectively. All but one inducible clindamycin resistant MRSA isolates were susceptible to clindamycin by Vitek II system. Five inducible clindmycin resistant MSSA isolates were all susceptible to clindamycin by Vitek II system. Vitek II system did not detect the inducible clindamycin resistance in S. aureus. CONCLUSIONS: Our results showed that Vitek II system was unacceptable for the detection of inducible clindamycin resistance in S. aureus. We suggests that the disk approximation test should be used to detect the inducible clindamycin resistance in S. aureus.
