Immunohistochemical Study for the Syndecan-2 in Cutaneous Melanoma and Pigmented Nevus.
- Author:
Woo Jin YOON
1
;
So Ra CHOI
;
Tai Kyung NOH
;
Ho Young PARK
;
Jung Ran CHOI
;
Sung Eun CHANG
;
Mi Woo LEE
;
Jee Ho CHOI
Author Information
1. Department of Dermatology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea. cse@amc.seoul.kr
- Publication Type:Original Article
- Keywords:
Melanoma;
Syndecan-2
- MeSH:
Blotting, Western;
Cell Line;
Humans;
Melanocytes;
Melanoma*;
Nevus;
Nevus, Pigmented*;
Phenotype;
Polymerase Chain Reaction;
Syndecan-2*
- From:Korean Journal of Dermatology
2013;51(10):776-781
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Melanoma is a malignant neoplasm originating from melanocytes. It has been recently suggested that syndecan-2 may contribute to the aggressive phenotype and metastatic potential of melanoma in cell line studies. However, there is no quantitative analysis of syndecan-2 expression using human melanoma tissue. OBJECTIVE: This study aimed to examine the specific expression of syndecan-2 in human melanoma tissue. METHODS: A total of 35 sections of formalin-fixed, paraffin-embedded tissues were investigated for syndecan-2 expression using immunohistochemical staining. Also, a total of 6 tissues and two kinds of cell lines were analyzed by reverse-transcriptase polymerase chain reaction (RT-PCR) and western blot. RESULTS: Immunohistochemical staining of 23 cases of melanoma tissue was done, and in 5 cases (21.7%), strong expression of syndecan-2 was seen. Also, syndecan-2 was detected in human melanoma tissue and MNT-1 melanoma cells by RT-PCR and western blot analysis. CONCLUSION: We suggest that syndecan-2 expression is increased in melanoma compared to nevus. The results of this study may help to explain the clinical features of melanoma and syndecan-2.
