Effects of Cyclosporin on Pterygium Fibroblasts.
10.3341/jkos.2012.53.3.466
- Author:
Jong Soo LEE
1
;
Seung Wook LEE
;
Sang Jun LEE
;
Na Mi KIM
Author Information
1. Department of Ophthalmology, Pusan National University College of Medicine, Busan, Korea. jongsool@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
Cyclosporin;
IL;
LDH;
MMP;
Pterygium
- MeSH:
Cell Proliferation;
Cyclosporine;
Enzyme-Linked Immunosorbent Assay;
Fibroblasts;
Humans;
Interleukin-6;
Interleukin-8;
L-Lactate Dehydrogenase;
Laminin;
Pterygium;
Recurrence;
Tumor Necrosis Factor-alpha
- From:Journal of the Korean Ophthalmological Society
2012;53(3):466-472
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To evaluate the response and cellular damage of cultured human pterygial cells according to the concentration and exposure time of topical cyclosporin. METHODS: Human pterygial cells were exposed to a cyclosporin A concentrations of 0.1 microg/ml (0.0001%), 1 microg/ml (0.0001%), 10 microg/ml (0.001%), 100 microg/ml (0.01%), or 500 microg/ml (0.05%) for 5 or 10 minutes. An MTT-based colorimetric assay was performed to assess the metabolic activity of cellular proliferation, and a lactate dehydrogenase (LDH) leakage assay was used to determine cellular damage. The extra-cellular matrix of PIP, laminin and MMP were evaluated, and the measurement of pro-inflammatory cytokine, TNF-alpha and IL-1b. IL-6, IL-8 was performed using ELISA kits. RESULTS: The pterygial cellular inhibitory effect of cyclosporin was similar to that of the control according to the concentration and exposure time (p > 0.05). Compared with the control, the level of LDH did not show a statistically significant difference between concentration and exposure time (p > 0.05). There was no significant difference of inhibitory effects by PIP, laminin, or MMP between the experimental and control groups (p > 0.05). The production of TNF-alpha and IL from the experimental pterygial cells due to the effect of cyclosporin was not significantly different from that of the control at a longer exposure time or stronger concentration (p > 0.05). CONCLUSIONS: The response of pterygial cells to topical cyclosporin A at concentrations less than 0.05% for less than 10 minutes of exposure time showed no prevention of pterygial recurrence. With regard to cellular damage, little effects on inhibition of PIP, laminin, MMP, IL, and TNF-alpha were observed compared with that of the control.
