Non-Surgical Gene Transfer to Rabbit Renal Glomeruli via Percutaneous Arterial Catheterization.
- Author:
Byeong Ho PARK
1
;
Tae Ho HWANG
;
Seung Eun KIM
;
Ki Hyun KIM
;
Geun Soo KONG
;
Ho Sun JUN
;
Jin Sook JEONG
;
Seo Hee RHA
Author Information
1. Department of Radiology, Dong-A University College of Medicine, Busan, Korea.
- Publication Type:Original Article
- Keywords:
Glomerulus;
Gene transfer;
Adenovirus
- MeSH:
Adenoviridae;
Animals;
beta-Galactosidase;
Catheterization*;
Catheters*;
Cytomegalovirus;
Endothelial Cells;
Genetic Therapy;
Humans;
Mesangial Cells;
Pathology;
Renal Artery
- From:Korean Journal of Nephrology
2005;24(1):19-25
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Transfer of foreign genes to the renal glomerular cells is an important step for the gene therapy of renal diseases in which the primary pathology is confined to the glomeruli. We developed a non-surgical method of gene transfer to rabbit renal glomeruli using percutaneous arterial catheterization without any laparatomy procedure. METHODS: The recombinant adenovirus type 5, containing a nuclear-targeted beta-galactosidase gene and driven by a cytomegalovirus promoter, was slowly infused into the unilateral renal artery via percutaneous arterial catheterization. The animals were sacrificed 3 days after virus infusion and lacZ staining was done on the fresh harvested tissue. RESULTS: Only the animals those received 6x10(12) particles/rabbit for 120 minutes show lacZ expression in 90.6+/-5% (n=3) of glomeruli. Mostly, it was the endothelial cells and mesangial cells those were positive for the stain. CONCLUSION: This non-surgical method for gene transduction of the renal glomeruli can be applied to human trials of glomerulus-directed gene therapy.
