Fasting-induced Down-regulation of NADPH-diaphorase in the Magnocellular PVN of Rats.
10.3349/ymj.2004.45.5.917
- Author:
Joo Young LEE
1
;
Dong Won KANG
;
Dong Goo KIM
;
Jeong Won JAHNG
Author Information
1. Department of Pharmacology, BK21 Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea. jwjahng@yumc.yonsei.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Food deprivation;
hypothalamus;
neuronal nitric oxide synthase;
glucocorticoid
- MeSH:
Adrenalectomy;
Animals;
Dexamethasone/pharmacology;
Down-Regulation;
Fasting/*metabolism;
Male;
NADPH Dehydrogenase/*analysis;
Nitric-Oxide Synthase/genetics;
Paraventricular Hypothalamic Nucleus/*enzymology;
Peptides/physiology;
Rats;
Rats, Sprague-Dawley;
Research Support, Non-U.S. Gov't;
Weight Gain
- From:Yonsei Medical Journal
2004;45(5):917-922
- CountryRepublic of Korea
- Language:English
-
Abstract:
In this study, we examined if glucocorticoids are required for the fasting-induced decrease of neuronal nitric oxide synthase (nNOS) in the magnocellular division of the paraventricular nucleus (PVN). Rats were adrenalectomized, subjected to 48 h of food deprivation with/without dexamethasone (5 mg/ kg, 4 subcutaneous injections with 12 h intervals), and the brain slices were processed for NADPH-diaphorase (NADPH- d) staining, a histochemical marker for nNOS in neuronal cells. In food deprived adrenalectomized rats, but not in free fed intact rats, dexamethasone significantly decreased NADPH-d staining in the magnocellular PVN. We previously reported that food deprivation decreases nNOS in the magnocellular PVN of intact rats. Thus, the present results together with our previous report suggest that although glucocorticoids are required for fasting-induced nNOS down-regulation in the magnocellular PVN, glucocorticoids may not be directly involved and some other molecular signals produced by food deprivation may play a pivotal role over glucocorticoid in the regulatory pathway for nNOS expression in this brain region.
