Osteoprotegerin is present on the membrane of osteoclasts isolated from mouse long bones.
- Author:
Kyung Mi WOO
1
;
Youngnim CHOI
;
Seong Hee KO
;
Jea Seung KO
;
Kwi Ok OH
;
Kack Kyun KIM
Author Information
1. College of Dentistry, Kangnung National University, Kangung, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Bone and Bones;
cell membrane;
cytokine receptors;
mice;
osteoclast;
tumor necrosis factor
- MeSH:
Animals;
Bone and Bones/cytology/*metabolism;
Carrier Proteins/immunology/metabolism;
Cell Differentiation/drug effects/physiology;
Cell Membrane/*metabolism;
Cells, Cultured;
Glycoproteins/drug effects/*metabolism;
Macrophage Colony-Stimulating Factor/pharmacology;
Membrane Glycoproteins/immunology/metabolism;
Mice;
Mice, Inbred ICR;
Osteoclasts/drug effects/*metabolism;
Receptors, Cytoplasmic and Nuclear/drug effects/*metabolism;
Stem Cells/drug effects/metabolism
- From:Experimental & Molecular Medicine
2002;34(5):347-352
- CountryRepublic of Korea
- Language:English
-
Abstract:
Osteoprotegerin (OPG), a member of the tumor necrosis factor receptor superfamily, is known to inhibit osteoclastogenesis by acting as a soluble decoy receptor for the receptor activator of NF-kB ligand (RANKL). We report the presence of OPG on the membrane of osteoclasts and the possibility of the direct action of OPG on them. Highly pure osteoclast precursors were isolated from mouse long bones and induced to differentiate into mature osteoclasts by M-CSF and soluble RANKL (sRANKL). The presence of OPG on the membrane of these cells was confirmed by western blotting and immunostaining. Furthermore, sRANKL was found to be bound to the OPG on the osteoclast precursors. These results suggest that OPG might have a new role during the differentiation of osteoclasts beyond its role as a soluble decoy receptor. The mechanism of the existence of OPG on osteoclast precursors remains to be found.
