Proteomic analysis of the cellular proteins induced by adaptive concentrations of hydrogen peroxide in human U937 cells.
- Author:
Je Kyung SEONG
1
;
Do Kyun KIM
;
Kun Ho CHOI
;
Seung Hyun OH
;
Kil Soo KIM
;
Seung Sook LEE
;
Hong Duck UM
Author Information
1. Laboratory of Developmental Biology, College of Veterinary Medecine, Seoul National University, Seoul, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Biological adaptation;
cell death;
hydrogen peroxide;
oxidative stress;
proteomics
- MeSH:
Adaptation, Physiological/drug effects/*physiology;
Cells, Cultured;
Human;
Hydrogen Peroxide/*pharmacology;
Proteins/drug effects/*metabolism;
Proteomics/*methods;
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization;
U937 Cells
- From:Experimental & Molecular Medicine
2002;34(5):374-378
- CountryRepublic of Korea
- Language:English
-
Abstract:
When cells are first exposed to low levels of oxidative stress, they develop a resistance to a subsequent challenge of the same stress, even at higher levels. Although some protein(s) induced by oxidative stress likely mediated this adaptive response, the nature of these proteins is unknown. In this study, the total proteins extracted from human U937 leukemia cells exposed to 50 mM H2O2 for 24 h to induce an optimal protective response were analyzed by two-dimensional polyacrylamide gel electrophoresis. H2O2 treatment induced elevation of level of 34 protein spots. An analysis of these spots by a matrix associated laser desorption/ionization time-of-flight mass spectrometry identified 28 of the H2O2-induced proteins. These include proteins involved in energy metabolism, translation and RNA processing, chaperoning or mediating protein folding, cellular signaling, and redox regulation, as well as a mitochondrial channel component, and an actin-bundling protein. Therefore, it appears that the cellular adaptation to oxidative stress is a complex process, and is accompanied by a modulation of diverse cellular functions.
