A Comparative Study of Gene Microarray between Normal and Diabetic Keratocyte in Rat Cornea.
- Author:
Jong Soo LEE
1
;
Joo Rak LEE
;
Suk Chul JUNG
;
Boo Sup OUM
Author Information
1. Department of Ophthalmology, Pusan National University College of Medicine, Pusan, Korea. jongsool@pusan.ac.kr
- Publication Type:Comparative Study ; Original Article
- Keywords:
Atlas(TM) Glass Microarrays 1.0;
Diabetic keratocyte;
Hybridization
- MeSH:
Animals;
Cornea*;
Electron Transport Complex IV;
Glass;
Glypicans;
GTP-Binding Proteins;
Ion Channels;
Oncogenes;
Proteasome Endopeptidase Complex;
Protein Kinases;
Rats*;
Stromal Cells
- From:Journal of the Korean Ophthalmological Society
2003;44(7):1629-1638
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To differentiate the genes that are up-regulated or down-regulated and the cellular morphology in the corneal stromal cells between normal and diabetic rat. METHODS: After isolation with trizol (GIBCO, NY, USA), labeling and purification of complementary DNA(cDNA) probes were carried out using Atlas(TM) Glass Microarrays 1.0. RESULTS: Autoradiographic analysis showed that 35 genes were up-regulated and 8 genes were down-regulated by Atlas Image(TM) Software. Up-regulated genes were as calcium/calmodulin dependent protein kinase I, proteasome 26S subunit 1, prostaglandin F receptor. Down-regulated genes were aquaporin 8, cytochrome c oxidase polypeptide Vb, glypican 1. CONCLUSIONS: There were many differential genes in hormone receptor, voltage gated ion channels, oncogenes and tumor suppressors, G protein between normal and diabetic keratocyte of rat.
