The Effect of RGP Lens and Reverse Geometry Lens on Apoptosis in Rabbit Cornea.
- Author:
Dong Bin SHIN
1
;
Jung Chul KIM
;
Mee Kum KIM
;
Kil Whan KO
;
Jae Lim LEE
;
Si Whan CHOI
Author Information
1. Department of Ophthalmology, Chungnam National University College of medicine, Daejon, Korea. opht@unitel.co.kr
- Publication Type:Original Article
- Keywords:
Apoptosis;
Reverse geometry lens;
Rigid gas permeable lens;
TUNEL stain
- MeSH:
Apoptosis*;
Cornea*;
Corneal Stroma;
Epithelial Cells;
Epithelium, Corneal;
Fibroblasts;
In Situ Nick-End Labeling;
Microscopy, Electron, Transmission;
Rabbits
- From:Journal of the Korean Ophthalmological Society
2003;44(7):1649-1661
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To investigate the cytotoxic effect of rigid gas permeable(RGP) and reverse geometry lens(RGL) on rabbit corneal cells. METHODS: In 36 white rabbits, right eyes were worn with either RGP or RGL. Left eyes were served as control. The rabbits were sacrificed at 1, 3, 7, 14 days after fitting and 7, 14 days after removal. Morphological changes of the basal epithelial cells and stromal keratocytes were observed using the light and transmission electron microscopy. The samples were examined after TUNEL staining in 3 high power fields (x400). RESULTS: In control eyes, 2.26% of central corneal epithelial cells showed apoptosis. After RGP wearing, the epithelial apoptosis decreased by 0.32% compared to control eyes(2.17%) at 14 days(p<0.001), and returned to 1.55% at 14 days after lens removal(2.04% in control, p=0.161). After RGL wearing, the degree of apoptosis was also lower than control(3.22%) by 0.30% at 14 days(p=0.003). However, it increased to 0.75% at 14 days after lens removal(1.67% in control, p=0.077). A few TUNEL positive fibroblasts were found in superficial stroma in both RGP and RGL group, but not in control eyes. CONCLUSIONS: Both RGP and RGL wearing may decrease the degree of apoptosis in corneal epithelium and lead the apoptosis in corneal stroma. The degree of apoptosis was recovered after lens removal, suggesting the process might to be reversible. The fitting with RGL induced apoptosis of keratocyte more than the fitting with RGP did. It suggests that the RGL wearing may be less physiologic than RGP wearing.
