Enhancement of Gastric Ulcer Healing and Angiogenesis by Cochinchina Momordica Seed Extract in Rats.
10.3346/jkms.2010.25.6.875
- Author:
Jung Mook KANG
1
;
Nayoung KIM
;
Bongcheol KIM
;
Joo Hyon KIM
;
Bong Yong LEE
;
Ji Hyun PARK
;
Mi Kyoung LEE
;
Hye Seung LEE
;
Joo Sung KIM
;
Hyun Chae JUNG
;
In Sung SONG
Author Information
1. Department of Internal Medicine and Liver Research Institute, Seoul National University College of Medicine, Seoul, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Cochinchina Momordica Seed Extract;
Acetic Acid;
Stomach Ulcer;
Vascular Endothelial Growth Factor;
Angiogenesis
- From:Journal of Korean Medical Science
2010;25(6):875-881
- CountryRepublic of Korea
- Language:English
-
Abstract:
Cochinchina momordica seed is the dried ripe seed of Momordica cochinchinensis, a perennial vine. The antiulcer effect of an extract from cochinchina momordica seeds (SK-MS10) was evaluated in a rat model of acetic acid-induced gastric ulcers. Gastric ulcers were produced by subserosal injection of acetic acid. SK-MS10 (200 mg/kg) or vehicle was administered orally once per day for 14 days after the acetic acid injection. The stomach was removed and the ulcer size measured at day 7 and 14 of the treatment. Expression of vascular endothelial growth factor (VEGF) was assessed by real-time RT-PCR and Western blot analysis. In addition, the microvasculature density (MVD) adjacent to the ulcer margin was examined by immunohistochemistry. The treatment with SK-MS10 for 7 and 14 days significantly accelerated ulcer healing and increased the expression of mRNA (at day 7) as well as VEGF protein (at day 14) compared to the vehicle-treated rats. The MVD for factor VIII was also higher in the SK-MS10 treatment group compared to the vehicle-treated rats; however, these differences were not statistically significant. These results suggest that SK-MS10 treatment accelerates the healing of gastric ulcers via upregulation of VEGF and angiogenesis in an acetic acid rat model.
