Expression of Cell Surface Receptors on Human Glioblastoma Xenograft Model in NOD/SCID Mouse.
- Author:
Kyung Seung OH
1
;
Ki Uk KIM
;
Na Hee PARK
;
Su Yeong SEO
;
Sun Seob CHOI
;
Gi Yeong HUH
Author Information
1. Department of Diagnostic Radiology, Kosin University College of Medicine, Busan, Korea.
- Publication Type:Original Article
- Keywords:
Glioblastoma;
Interleukin-4 receptor;
SCID mouse
- MeSH:
Animals;
Antibodies;
Cell Line;
Endothelial Cells;
Glioblastoma*;
Heterografts*;
Humans*;
Immunotoxins;
Mice*;
Mice, SCID;
Necrosis;
Receptors, Cell Surface*
- From:Cancer Research and Treatment
2002;34(1):52-57
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To obtain basic data for development of a glioblastoma-specific immunotoxin, the expression of variable cell surface receptors on a human glioblastoma xenograft model was evaluated, using NOD/SCID mice. MATERIALS AND METHODS: We developed a xenograft model in NOD/SCID mice implanted with a human glioblastoma cell line (U-87MG). Immunohistochemical studies were performed on implanted tumor nodules (n=8) using antibodies against CD71, EGFR, IGF-IRalpha, CXCR4 and IL-4Ralpha. RESULTS: Expression of IL-4Ralpha, in implanted tumornodules, was the highest of the cell surface receptors evaluated in this study. However, the endothelial cells in, and around, the tumor nodules also revealed immunopositivity against IL-4Ralpha. The immunoreactivity of IL-4Ralpha, and other surface receptors such as CD71, IGF-IRalpha and EGFR, was prominent in tumor nodules associated with tumor necrosis. CONCLUSION: IL-4Ralpha would be a possible target for the development of glioblastoma-specific immunotoxin, although there are limitations due to its endothelial expression.
