Trichomonas vaginalis α-Actinin 2 Modulates Host Immune Responses by Inducing Tolerogenic Dendritic Cells via IL-10 Production from Regulatory T Cells.
10.3347/kjp.2017.55.4.375
- Author:
Hye Yeon LEE
1
;
Juri KIM
;
Jae Sook RYU
;
Soon Jung PARK
Author Information
1. Department of Environmental Medical Biology and Institute of Tropical Medicine, Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul 03722, Korea. sjpark615@yuhs.ac
- Publication Type:Original Article
- Keywords:
Trichomonas vaginalis;
α-actinin 2;
Treg cell;
interleukin-10 (IL-10)
- MeSH:
Animals;
Antibodies;
Blotting, Western;
Dendritic Cells*;
Enzyme-Linked Immunosorbent Assay;
Epithelial Cells;
Humans;
Interleukin-10*;
Mice;
Prostate;
T-Lymphocytes;
T-Lymphocytes, Regulatory*;
Trichomonas vaginalis*;
Trichomonas*
- From:The Korean Journal of Parasitology
2017;55(4):375-384
- CountryRepublic of Korea
- Language:English
-
Abstract:
Trichomonas vaginalis is a pathogen that triggers severe immune responses in hosts. T. vaginalis α-actinin 2, Tvα-actinin 2, has been used to diagnose trichomoniasis. This study was undertaken to examine the role of Tvα-actinin 2 as an antigenic molecule to induce immune responses from humans. Western blot analysis using anti-Tvα-actinin 2 antibodies indicated its presence in the secreted proteins of T. vaginalis. ELISA was employed to measure cytokine production by vaginal epithelial cells, prostate cells, mouse dendritic cells (DCs), or T cells stimulated with T. vaginalis or Tvα-actinin 2 protein. Both T. vaginalis and rTvα-actinin 2 induced cytokine production from epithelial cell lines, including IL-10. Moreover, CD4+CD25− regulatory T cells (Treg cells) incubated with rTvα-actinin 2-treated DCs produced high levels of IL-10. These data indicate that Tvα-actinin 2 modulates immune responses via IL-10 production by Treg cells.
