Effect of a Flavon Extracted from Artemisia absinthium on Collagen Induced Arthritis in Mice.
- Author:
Kyong Min CHOI
1
;
Dae Hwan HWANG
;
Kyung Mi SHIN
;
Do Young YOON
;
Dong Soo KIM
Author Information
1. Department of Pediatrics, Yonsei University College of Medicine, Seoul, Korea. dskim6634@yumc.yonsei.ac.kr
- Publication Type:Original Article
- Keywords:
Flavon;
Artemisia absinthium;
Collagen induced arthritis;
IL-10;
TNF-alpha
- MeSH:
Animals;
Antibodies;
Artemisia absinthium*;
Artemisia*;
Arthritis*;
Arthritis, Experimental;
Chickens;
Collagen Type II;
Collagen*;
Enzyme-Linked Immunosorbent Assay;
Immunization;
Incidence;
Interleukin-10;
Mice*;
RNA, Messenger;
Tumor Necrosis Factor-alpha
- From:Korean Journal of Pediatrics
2004;47(6):677-684
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: In this study, a possible suppressive effect of a flavon extracted from Artemisia absinthium on a mouse collagen-induced arthritis (CIA) model was investigated. METHODS: DBA/1 mice were injected intradermally with emulsified chicken type II collagen. Three weeks after immunization, a flavon was introduced p.o. everyday. Clinical incidences of arthritis and arthritis index were measured. Measurement of anti-collagen antibodies and a stimulation index of the splenocytes of the mice were measured. IL-10 and TNF-alpha in the supernatants of the mice sera were measured by ELISA. mRNA expression for IL-10 and TNF-alpha in the splenocytes were tested. RESULTS: Flavon extracted from Artemisia absinthium appears to be an effective suppressor of CIA in mice. The serum anti-collagen antibody level and stimulation index of the cultured splenocytes showed no significant differences among the three experimental groups. Also serum IL-10 and TNF-alpha levels did not show any significant differences among the three experimental groups. An increased expression of mRNA for IL-10 was observed in the splenocytes treated with flavon. CONCLUSION: With these results, flavon extracted from Artemisia absinthium appears to have a suppressive effect of CIA. The mechanism of the suppressive effect of flavon extracted from Artemisia absinthium may be from a stimulation of IL-10 production.
