Cardioprotective Effects of Minocycline in Rat Myocardiac Cell Culture Induced by Hypoxia.
- Author:
Bum Seok YANG
1
;
Hye Jin PARK
;
Hong Tae KIM
;
Jong Won LEE
;
Dong Suk LEE
;
Un Seok NHO
;
Hai Lee CHUNG
;
Woo Taek KIM
Author Information
1. Department of Pediatrics, School of Medicine, Catholic University of Daegu, Daegu, Korea. wootykim@cu.ac.kr
- Publication Type:Original Article
- Keywords:
Minocycline;
Hypoxia;
Myocardial infarction;
Myocardiac cells;
Cultured
- MeSH:
Animals;
Anoxia*;
Apoptosis;
Cell Culture Techniques*;
Cells, Cultured;
Heart;
Humans;
Minocycline*;
Mortality;
Myocardial Infarction;
Rats*;
Rats, Sprague-Dawley
- From:Korean Journal of Pediatrics
2004;47(6):685-690
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Minocycline appears to reduce the mortality rate of patients with myocardial infarction. Therefore, the cardioprotective effects of minocycline through reduction of apoptosis were studied in a rat myocardiac cell culture induced by hypoxia. METHODS: Cultured cells from the hearts of Sprague-Dawley rats were divided into two sets of groups: normoxia groups treated with 5% O2 and hypoxia groups treated with 1% O2. After several days of incubation, the control groups were not treated with minocycline, while the sample groups were treated with 1 mug/mL of minocycline. The damaged cells were observed under a microscope, while apoptosis was detected using a Tdt-mediated dUTP nick and labeling(TUNEL) assay control-stained with 4'-6'-diamidino-2-phenylindole-2HCl(DAPI). RESULTS: In normoxia, the control group showed a statistically significant difference from the sample group treated with 1 mug/mL of minocycline. In addition, in hypoxia, there was also a statistically significant difference between the two groups. When comparing the normoxia and hypoxia groups to each other, there was a statistically significant difference between the control groups and sample groups. CONCLUSION: Minocycline was found to be protective for heart in normoxia and in hypoxia in the model of rat myocardiac cell culture.
