Oral immunization of mice with recombinant rabies vaccine strain (ERAG3G) induces complete protection.
10.7774/cevr.2015.4.1.107
- Author:
Dong Kun YANG
1
;
Ha Hyun KIM
;
Sung Suk CHOI
;
Jong Taek KIM
;
Woong Ho JEONG
;
Jae Young SONG
Author Information
1. Viral Disease Division, Animal and Plant Quarantine Agency, MAFRA, Anyang, Korea. yangdk@korea.kr
- Publication Type:Original Article
- Keywords:
Recombinant rabies virus;
Reverse genetics;
Mouse;
Animals
- MeSH:
Administration, Oral;
Animals;
Antibodies, Neutralizing;
Body Weight;
Cats;
Genome;
Glycoproteins;
Immunization*;
Korea;
Mice*;
Plasmids;
Rabies;
Rabies Vaccines*;
Rabies virus;
Raccoon Dogs;
Reverse Genetics
- From:Clinical and Experimental Vaccine Research
2015;4(1):107-113
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: New rabies vaccine bait for both pets and raccoon dogs residing in Korea is needed to eradicate rabies infection among animals. In this study, we constructed a recombinant rabies virus (RABV), the ERAG3G strain, using a reverse genetics system. Then we investigated the efficacy of this strain in mice after oral administration and the safety of this strain in cats after intramuscular administration. MATERIALS AND METHODS: The ERAG3G strain was rescued in BHK/T7-9 cells using the full-length genome mutated at the amino acid position 333 of the glycoprotein gene of RABV and helper plasmids. Four-week-old mice underwent one or two oral administrations of the ERAG3G strain and were challenged with the highly virulent RABV strain CVSN2c 14 days after the second administration. Clinical symptoms were observed and body weights were measured every day after the challenge. RESULTS: All mice showed complete protection against virulent RABV. In addition, cats intramuscularly inoculated with the ERAG3G strain showed high antibody titers ranging from 2.62 to 23.9 IU/mL at 28-day postinoculation. CONCLUSION: The oral immunization of the ERAG3G strain plays an important role in conferring complete protection in mice, and intramuscular inoculation of the ERAG3G strain induces the formation of anti-rabies neutralizing antibody in cats.
