The effect of rhBMP-2 on the osteoblastic differentiation of human periodontal ligament cells and gingival fibroblasts in vitro.
10.5051/jkape.2002.32.2.389
- Author:
Hyeon Jong KIM
1
;
Sang Mook CHOI
;
Young KU
;
In Chul RHYU
;
Chong Pyoung CHUNG
;
Soo Boo HAN
;
kyoung Hwa KIM
;
Yong Moo LEE
Author Information
1. Department of Periodontology, College of Dentistry, Seoul National University, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
recombinant human bone morphogenetic protein-2(rhBMP-2);
periodontal ligament cell;
osteoblast;
differentiation
- MeSH:
Actins;
Alkaline Phosphatase;
Animals;
Bone Matrix;
Cell Count;
Cell Proliferation;
Cells, Cultured;
Collagen Type I;
Fibroblasts*;
Humans*;
Integrin-Binding Sialoprotein;
Osteoblasts*;
Osteocalcin;
Osteogenesis;
Osteopontin;
Periodontal Ligament*;
Rats;
RNA;
RNA, Messenger
- From:The Journal of the Korean Academy of Periodontology
2002;32(2):389-401
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BMP can induce ectopic bone formation when implanted into sites such as rat muscle and can greatly enhance healing of bony defects when applied exogenously. In addition, BMP stimulated osteoblastic differentiation in vitro in various types of cells. The aim of this study was to investigate the effect of recombinant human bone morphogenetic protein(rhBMP-2) on the proliferation and osteoblastic differentiation of human periodontal ligament cells and gingival fibroblasts. The cell number and alkaline phosphatase activity were measured in 3 experimental groups of human periodontal ligament cells and gingival fibroblasts(control group, rhBMP-2 50ng/ml group, and rhBMP-2 100ng/ml group) at 1 and 2 weeks after culture. At the same time, total RNA of cultured cells were extracted and reverse trascription polymerase chain reaction(RT-PCR) was performed to determine the expression of mRNA of bone matrix protein. RhBMP-2 had no effect on the cell proliferation of human periodontal ligament cells and gingival fibroblasts. Alkaline phosphatase activity was elevated significantly by rhBMP-2 in both cells. And periodontal ligament cells showed significantly higher alkaline phosphatase activity than gingival fibroblasts. beta-actin, type I collagen, alkaline phosphatase, BMP-2 mRNA were expressed in all of the samples. Osteopontin, osteocalcin mRNA were expressed in all periodontal ligament cell groups, and rhBMP-2 50ng/ml group, rhBMP-2 100ng/ml group of 2 week culture period of gingival fibroblasts. Bone sialoprotein mRNA was only expressed in rhBMP-2 50ng/ml group and rhBMP-2 100ng/ml group of 2-week culture period. These results suggest that rhBMP-2 stimulates osteoblastic differentiation in human periodontal ligament cells and gingival fibroblasts in vitro.
