Molecular characterization of the feline T-cell receptor gamma alternate reading frame protein (TARP) ortholog.
10.4142/jvs.2012.13.4.345
- Author:
Alexander Th A WEISS
1
;
Marie Charlotte VON DEETZEN
;
Werner HECHT
;
Manfred REINACHER
;
Achim D GRUBER
Author Information
1. Institute of Veterinary Pathology, Justus-Liebig-Universitat Giessen, 35392 Giessen, Germany. alexander.weiss@cvua-mel.de
- Publication Type:Original Article
- Keywords:
cat;
C-region;
J-region;
T-cell receptor gamma alternate reading frame protein (TARP);
tumor marker
- MeSH:
Animals;
Cats;
Continental Population Groups;
Humans;
Immunotherapy;
Models, Animal;
Open Reading Frames;
Prostate;
Prostatic Neoplasms;
Protein Sorting Signals;
Pseudogenes;
Reading Frames;
Receptors, Antigen, T-Cell;
Recombination, Genetic;
Spleen
- From:Journal of Veterinary Science
2012;13(4):345-353
- CountryRepublic of Korea
- Language:English
-
Abstract:
T-cell receptor gamma alternate reading frame protein (TARP) is expressed by human prostate epithelial, prostate cancer, and mammary cancer cells, but is not found in normal mammary tissue. To date, this protein has only been described in humans. Additionally, no animal model has been established to investigate the potential merits of TARP as tumor marker or a target for adoptive tumor immunotherapy. In this study conducted to characterize feline T-cell receptor gamma sequences, constructs very similar to human TARP transcripts were obtained by RACE from the spleen and prostate gland of cats. Transcription of TARP in normal, hyperplastic, and neoplastic feline mammary tissues was evaluated by conventional RT-PCR. In felines similarly to the situation reported in humans, a C-region encoding two open reading frames is spliced to a J-region gene. In contrast to humans, the feline J-region gene was found to be a pseudogene containing a deletion within its recombination signal sequence. Our findings demonstrated that the feline TARP ortholog is transcribed in the prostate gland and mammary tumors but not normal mammary tissues as is the case with human TARP.
