Application of a multiplex PCR assay for Campylobacter fetus detection and subspecies differentiation in uncultured samples of aborted bovine fetuses.
10.4142/jvs.2012.13.4.371
- Author:
Gregorio IRAOLA
1
;
Martin HERNANDEZ
;
Lucia CALLEROS
;
Fernando PAOLICCHI
;
Silvia SILVEYRA
;
Alejandra VELILLA
;
Luis CARRETTO
;
Eliana RODRIGUEZ
;
Ruben PEREZ
Author Information
1. Evolutionary Genetics Section, Department of Animal Biology, Faculty of Sciences, Institute of Biology, University of the Republic, 11400 Montevideo, Uruguay. rperez@fcien.edu.uy
- Publication Type:Original Article
- Keywords:
bovine aborted fetuses;
Campylobacter fetus subspecies venerealis;
multiplex PCR
- MeSH:
Animals;
Campylobacter;
Campylobacter fetus;
Cattle;
Communicable Diseases;
Fetus;
Multiplex Polymerase Chain Reaction;
Pathology, Molecular;
Sheep
- From:Journal of Veterinary Science
2012;13(4):371-376
- CountryRepublic of Korea
- Language:English
-
Abstract:
Campylobacter (C.) fetus (epsilonproteobacteria) is an important veterinary pathogen. This species is currently divided into C. fetus subspecies (subsp.) fetus (Cff) and C. fetus subsp. venerealis (Cfv). Cfv is the causative agent of bovine genital Campylobacteriosis, an infectious disease that leads to severe reproductive problems in cattle worldwide. Cff is a more general pathogen that causes reproductive problems mainly in sheep although cattle can also be affected. Here we describe a multiplex PCR method to detect C. fetus and differentiate between subspecies in a single step. The assay was standardized using cultured strains and successfully used to analyze the abomasal liquid of aborted bovine fetuses without any pre-enrichment step. Results of our assay were completely consistent with those of traditional bacteriological diagnostic methods. Furthermore, the multiplex PCR technique we developed may be easily adopted by any molecular diagnostic laboratory as a complementary tool for detecting C. fetus subspecies and obtaining epidemiological information about abortion events in cattle.
