Development of monoclonal antibodies against the abnormal prion protein isoform (PrPres) associated with chronic wasting disease (CWD).
10.4142/jvs.2012.13.4.429
- Author:
Hyun Jeong JEONG
1
;
Nak Hyung LEE
;
Joong Bok LEE
;
Seung Yong PARK
;
Chang Seon SONG
;
Kun Ho SEO
;
Dong Woon KIM
;
Yong Sun KIM
;
In Soo CHOI
Author Information
1. Department of Infectious Diseases, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Korea. ischoi@konkuk.ac.kr
- Publication Type:Brief Communication
- Keywords:
CWD;
elk;
monoclonal antibody;
prion;
PrPres
- MeSH:
Animals;
Antibodies, Monoclonal;
Blotting, Western;
Brain;
Mice;
Mice, Knockout;
Stress, Psychological;
Wasting Disease, Chronic
- From:Journal of Veterinary Science
2012;13(4):429-432
- CountryRepublic of Korea
- Language:English
-
Abstract:
Monoclonal antibodies (mAbs) specific for the abnormal prion protein isoform (PrPres) are indispensable for diagnosing chronic wasting disease (CWD). In this study, eight mAbs were developed by immunizing PrP knockout mice with recombinant elk PrP and an immunogenic PrP peptide. The reactivity of the mAbs to recombinant PrP and the PrP peptide was measured, and their isotypes were subsequently determined. Among them, four mAbs (B85-05, B85-08, B85-12, and B77-75) were shown by Western blotting to recognize proteinase K-treated brain homogenate derived from an elk suffering from CWD.
