Unusual mtDNA sequencing results from ancient DNA.
- Author:
Seung Bum SEO
1
;
Chong Min CHOUNG
;
Aihua ZHANG
;
Byoung Su JANG
;
Seong Ho YOO
;
Soong Deok LEE
Author Information
1. Department of Forensic Medicine, Seoul National University College of Medicine, Korea. sdlee@snu.ac.kr
- Publication Type:Original Article
- Keywords:
mtDNA;
ancient DNA;
degradation
- MeSH:
Adult;
DNA*;
DNA, Mitochondrial*;
Hair;
Humans;
Sequence Analysis;
Sequence Analysis, DNA;
Tooth
- From:Korean Journal of Legal Medicine
2007;31(1):36-40
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Sequence analysis of human mitochondrial DNA(mtDNA) is being used widely to characterize individual identification, particularly when there is insufficient nuclear DNA in samples for typing. Hair shafts, bones, teeth and other samples that are severely decomposed may be subjected to mtDNA analysis. As sample decomposes, however, the possibility of mtDNA to be degraded becomes high and the possibility of spurious results becomes high. In this case mtDNA sequencing results must be carefully analyzed. We got unusual results while typing two human bone samples, which were not compatible with human mtDNA sequence. Bones were about 50 and 35 years old. We report the results with discussions about ancient DNA sequencing.
