Immunohistochemical Study of the Expression of Bone Morphogenetic Protein(BMP-7) Following Regenerative Periodontal Surgery.
10.5051/jkape.2000.30.1.51
- Author:
Kyung Yoon HAN
1
;
Se Yeol CHO
;
Ki Jung LIM
;
Heung Jung KIM
;
Ju Cheol PARK
;
Byung Ock KIM
Author Information
1. Department of Periodontology, College of Dentistry, Chosun University, Korea.
- Publication Type:Original Article
- MeSH:
Bone Morphogenetic Protein 7;
Extracellular Matrix;
Matrix Metalloproteinases;
Periodontal Diseases;
Periodontium;
Regeneration
- From:The Journal of the Korean Academy of Periodontology
2000;30(1):51-63
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Extracellular matrix component is degraded by enzymes of thematrix metalloproteinases(MMPs). MMPs are produced by both hemopoietic and structural cells. Increased activity of MMP-3 in periodontium is strongly associated with inflammatory periodontal disease. The purpose of the present study was to estimate the effect of BMP-7 on regeneration of periodontium. The optical density was measured by microwell plate reader at 450 nm.The difference of the optical density and the relative activity ofMMP-3 according to the concentration were statistically analyzed by one way ANOVA. The results were as follows: 1.Tetracycline-HCl showed the tendency to inhibit the activity of MMP-3 at the concentration lower than 25microgram/ml. 2.Doxycycline-HCl inhibited significantly the activity of MMP-3 at the concentration lower than 100microgram/ml. 3.Minocycline-HCl inhibited the activity of MMP-3 at the concentration in the range of 10 to 200microgram/ml. Within the limit of the present study, the above results suggested that bone morphogenetic protein-7 may play a important role in development of periodontium.
