Smad6 Gene and Suppression of Radiation-Induced Apoptosis by Genistein in K562 cells.
- Author:
Soo Jin JEONG
1
;
Young Hee JIN
;
Yeo Jin YOO
;
Chang Ho DO
;
Min Ho JEONG
;
Gi Yeong HUH
;
Hye Ran BAE
;
Kwang Mo YANG
;
Chang Woo MOON
;
Sin Geun OH
;
Won Joo HUR
;
Hyung Sik LEE
Author Information
1. Department of Radiation Oncology, College of Medicine, Dong-A University, Pusan, Korea.
- Publication Type:Original Article
- Keywords:
PTK inhibitors;
Oncogene expression;
Radiation-induced apoptosis;
Smad6
- MeSH:
Apoptosis*;
Cell Line;
Dimethyl Sulfoxide;
DNA, Complementary;
Genistein*;
K562 Cells*;
Leukemia;
Particle Accelerators;
RNA, Messenger;
Sequence Analysis, DNA
- From:The Journal of the Korean Society for Therapeutic Radiology and Oncology
2001;19(3):245-251
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The genes involved on the suppression of radiation-induced apoptosis by genistein in K562 leukemia cell line was investigated. MATERIALS AND METHODS: K562 cells in exponential growth phase were irradiated with a linear accelerator at room temperature. Forx-ray irradiation and drug treatment, cultures were prepared at 2x105 cells/mL. The cells were irradiated with 10 Gy (Clinac 1800C, Varian, USA). Stock solutions of herbimycin A (HMA, Calbiochem, UK) and genistein (Calbiochem, UK) were prepared in dimethylsulfoxide (DMSO, Sigma, UK). After incubation at 37degreesC for 24 h, PCR-select cDNA subtractive hybridization, dot hybridization, DNA sequencing and Northern hybridization were examined. RESULTS: Smad6 gene was identified from the differentially expressed genes in K562 cells incubated with genistein which had been selected by PCR-select cDNA subtractive hybridization. The mRNA expression of Smad6 in K562 cells incubated with genistein was also higher than control group by Northern hybridization analysis. CONCLUSION: We have shown that Smad6 involved on the suppression of radiation-induced apoptosis by genistein in K562 leukemia cell line. It is plausible that the relationship between Smad6 and the suppression of radiation-induced apoptosis is essential for treatment development based on molecular targeting designed to modify radiation-induced apoptosis.
