Granulocyte-colony stimulating factor decreases the extent of ovarian damage caused by cisplatin in an experimental rat model.
10.3802/jgo.2014.25.4.328
- Author:
Ali AKDEMIR
1
;
Burak ZEYBEK
;
Levent AKMAN
;
Ahment Mete ERGENOGLU
;
Ahmet Ozgur YENIEL
;
Oytun ERBAS
;
Altug YAVASOGLU
;
Mustafa Cosan TEREK
;
Dilek TASKIRAN
Author Information
1. Department of Obstetrics and Gynecology, Ege University School of Medicine, Izmir, Turkey. burak.zeybek@ege.edu.tr
- Publication Type:Original Article
- Keywords:
Anti-Mullerian hormone;
Cisplatin;
Granulocyte-colony stimulating factor;
Ovarian follicle;
Ovary
- MeSH:
Animals;
Anti-Mullerian Hormone/blood;
Antineoplastic Agents/*toxicity;
Biological Markers/blood;
Cisplatin/*toxicity;
Disease Models, Animal;
Drug Evaluation, Preclinical/methods;
Female;
Fertility Preservation/methods;
Granulocyte Colony-Stimulating Factor/*therapeutic use;
Ovarian Follicle/drug effects/pathology;
Primary Ovarian Insufficiency/blood/chemically induced/pathology/*prevention & control;
Rats, Sprague-Dawley
- From:Journal of Gynecologic Oncology
2014;25(4):328-333
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: To investigate whether granulocyte-colony stimulating factor (G-CSF) can decrease the extent of ovarian follicle loss caused by cisplatin treatment. METHODS: Twenty-one adult female Sprague-Dawley rats were used. Fourteen rats were administered 2 mg/kg/day cisplatin by intraperitoneal injection twice per week for five weeks (total of 20 mg/kg). Half of the rats (n=7) were treated with 1 mL/kg/day physiological saline, and the other half (n=7) were treated with 100 microg/kg/day G-CSF. The remaining rats (n=7, control group) received no therapy. The animals were then euthanized, and both ovaries were obtained from all animals, fixed in 10% formalin, and stored at 4degrees C for paraffin sectioning. Blood samples were collected by cardiac puncture and stored at -30degrees C for hormone assays. RESULTS: All follicle counts (primordial, primary, secondary, and tertiary) and serum anti-Mullerian hormone levels were significantly increased in the cisplatin+G-CSF group compared to the cisplatin+physiological saline group. CONCLUSION: G-CSF was beneficial in decreasing the severity of follicle loss in an experimental rat model of cisplatin chemotherapy.
