Cardiac Hypertrophy in Transgenic Mice with Overexpressed Small-Subunit of Human Myosin Light Chain Phosphatase.
10.4070/kcj.2003.33.12.1165
- Author:
Sung Won BAE
1
;
Won Ha LEE
;
Chul Hong KIM
;
Haing Kee PARK
;
Sang Chol LEE
;
Young Hyeh KO
;
Seung Woo PARK
;
Takuro ARIMURA
;
Akinori KIMURA
;
Jeong Euy PARK
Author Information
1. Division of Cardiology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Cardiomyopathy, hypertrophic;
Phosphatase, myosin light chain;
Calcium sensitivity;
Mice, transgenic;
Protein subunits
- MeSH:
Animals;
Cardiomegaly*;
Cardiomyopathy, Hypertrophic;
Echocardiography;
Heart;
Humans*;
Hypertrophy;
Isoproterenol;
Mice;
Mice, Transgenic*;
Muscle Cells;
Myocardium;
Myosin Light Chains*;
Myosin-Light-Chain Phosphatase*;
Myosins*;
Phosphorylation;
Protein Subunits
- From:Korean Circulation Journal
2003;33(12):1165-1173
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: Recent reports have demonstrated that perturbation of the balance between myo-sin light chain (MLC) phosphorylation and the dephosphorylation status is associated with the development of cardiac hypertrophy. Myosin light chain phosphatase (MLCP) is a key enzyme that regulates the phosphorylation status of the MLC, but its functional roles in cardiac muscle have not been well investigated. Especially, the functions of the small-subunit of MLCP in cardiac muscles are not well elucidated. Here, whether the human heart-specific small-subunit (M21) of MLCP is associated with hypertrophic responses in a transgenic mice model were assessed. MATERIALS AND METHODS: The transgenic mice, overexpressing the human M21, were generated from a cardiac-specific transgenic construct. Cardiac tissues from the transgenic mice were subjected to histology for their morphological examination. The echocardiographic parameters of the murine heart were examined with transgenic mice aged 1, 2 and 3 months, and compared with their non-transgenic littermates. To determine whether the transgenic heart was sensitive to stress, the echocardiographic examination was also performed at the baseline, both before and after the administration of isoproterenol, at a dosage of 30 microgram/g/day, for 2 weeks. RESULTS: The histological analysis of the transgenic heart revealed myocyte disarray and nuclear hypertrophy. No significant differences were observed between the transgenic and non-transgenic mice in relation to the echocardiographic determinants, such as the left ventricular dimensions and the wall thickness. Chronic cardiac stress, induced by isoproterenol infusion, also failed to show any significant differences in relation to the same determinants. CONCLUSION: Overexpression of the human M21 in the murine heart induced myocyte hypertrophy. However, the overall cardiac functions were not affected under normal and stressed conditions.
