Deletion of Y-Chromosome Specific Genes in Testicular Germ Cell Tumor.
- Author:
Bong Ryoul OH
1
;
Geun Soo KIM
;
Sung Hun PARK
;
Mi Hwa KIM
;
Hyung Jin KIM
;
Chan CHOI
Author Information
1. Department of Urology, Chonnam National University Medical School, Gwangju, Korea. ohbr@chonnam.ac.kr
- Publication Type:Original Article
- Keywords:
Testicular tumor;
Y-chromosome;
Gene
- MeSH:
DNA;
Epithelial Cells;
Genome;
Germ Cells*;
Humans;
Neoplasms, Germ Cell and Embryonal*;
Polymerase Chain Reaction;
Testicular Neoplasms
- From:Korean Journal of Urology
2003;44(2):174-179
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: We hypothesized that the deletion of Y-chromosome specific genes is associated with testicular tumors. To test this hypothesis, we analyzed the expressions of five Y-chromosome specific genes in testicular tumor samples. MATERIALS AND METHODS: Thirty-five human testicular tumor specimens were processed for the micro-dissection of pure epithelial cells. The DNA was extracted, and polymerase chain reactions performed using five different specific primers (ZFY, DYS203, SMCYM45, GDB187507 and RH38676). These primers were designed based on sequences available in the public genome data bank. RESULTS: Deletion was observed in 88.6% of the testicular tumor specimens with at least one Y-chromosome specific gene. The losses of DYS203, ZFY, SMCYM45, RH38676 and GDB187507 were shown in 51.4, 42.9, 40, 28.6 and 20% of cases, respectively. There was a different pattern of loss of the Y-chromosome specific genes according to the histologic types of germ cell tumor. The losses of the DYS203 and GDB187507 genes were seen more frequently in the advanced stages. CONCLUSIONS: There was a significant loss of the Y-chromosome specific genes in testicular germ cell tumors. The loss of the DYS203 gene was observed in about half the cases, and was more frequent in the higher stages of testicular tumor. The loss of Y-chromosome specific genes is associated with testicular tumors, suggesting their role in the pathogenesis of this disease.
