Redox Regulating Protein APE1/Ref-1 Expression is Increased in Abdominal Aortic Coarctation-induced Hypertension Rats.
10.5646/jksh.2012.18.3.126
- Author:
Sun Heon SONG
1
;
Eun Jung CHO
;
Myoung Soo PARK
;
Yu Ran LEE
;
Hee Kyoung JOO
;
Gun KANG
;
Shin Kwang KANG
;
Sunga CHOI
;
Byeong Hwa JEON
Author Information
1. Department of Physiology, Chungnam National University School of Medicine, Daejeon, Korea. bhjeon@cnu.ac.kr
- Publication Type:Original Article
- Keywords:
Hypertension;
Aortic coarctation;
Kidney;
Endothelium;
Oxidative stress
- MeSH:
Acridines;
Animals;
Aorta;
Aortic Coarctation;
Arterial Pressure;
Blood Pressure;
Blotting, Western;
Endothelium;
Humans;
Hypertension;
Immunohistochemistry;
Kidney;
Lipid Peroxidation;
Luminescence;
Male;
Oxidation-Reduction;
Oxidative Stress;
Rats;
Rats, Sprague-Dawley;
Relaxation;
Salicylamides;
Superoxides
- From:Journal of the Korean Society of Hypertension
2012;18(3):126-135
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Aim of study is designed to investigate whether apurinic/apyrimidinic endonuclease-1/redox factor-1 (APE1/Ref-1) expression is changed in abdominal aortic coarctation models. METHODS: Male Sprague-Dawley rats were randomly assigned with abdominal aortic coarctation, repaired group, sham, and control groups. Endothelial function was assessed with endothelium-dependent relaxations. Detection of superoxide anion and lipid peroxidation was performed by lucigenin chemiluminescence and thiobarbituric acid-reactive substances assay. APE1/Ref-1 expression was measured with Western blot and immunohistochemistry. RESULTS: In anesthetized condition, the abdominal aortic coarctation rats showed hypertension as systolic/diastolic arterial pressure of 171/114 mm Hg, compared with 114/94 mm Hg of control. Endothelium-dependent relaxations were significantly impaired in the aortic coarctation which was recovered in 1 week after coarctation repair. Superoxide production and lipid peroxidation were elevated in aortic coarctation rats. In immunohistochemistry, APE1/Ref-1 expressions were increased at aorta and kidney in aortic coarctation rats. Increased APE1/Ref-1 expression in aorta was recovered by repair of coarctation. CONCLUSIONS: Taken together, it suggests that APE1/Ref-1 expression was increased in aortic coarctation-induced hypertensive rats, suggesting a biomarker for hypertension. Impaired endothelium dependent relaxation in the aortic coarctation can be modulated by repair of coarctation or the modulation of blood pressure.
